原发性肝细胞癌患者血清HBcrAg与组织中HBV DNA载量的关系  

Correlation between serum HBcr Ag and intracellular HBV DNA in patients with hepatocellular carcinoma

作　　者：李慧明[1] 贾健安 王蒙蒙[1] 顾星[1] 房萌[1] 高春芳[1] 

机构地区：[1]第二军医大学附属东方肝胆外科医院实验诊断科,上海200438

出　　处：《检验医学》2016年第11期948-952,共5页Laboratory Medicine

摘　　要：目的分析一种新型的乙型肝炎病毒(HBV)感染标志物——乙型肝炎核心相关抗原(HBcrAg)与原发性肝细胞癌(HCC)患者癌组织和癌旁组织内共价闭合环状DNA(cccDNA)、HBV DNA水平的关系。方法收集行肝癌切除术的HBV感染相关的HCC患者95例,用化学发光法检测患者血清HBcrAg水平,同时用聚合酶链反应(PCR)荧光探针法分别对配对的癌组织和癌旁组织内cccDNA、HBV DNA进行定量检测。结果乙型肝炎e抗原(HBe Ag)+/乙型肝炎e抗体(HBe Ab)-组、HBe Ag+/HBe Ab+组、HBe Ag-/HBe Ab+组HBcrAg水平分别为(6.41±1.18)、(5.80±0.50)和(5.16±0.94)lg U/m L,3个组间差异有统计学意义(P<0.001)。HCC患者HBcrAg与癌旁组织cccDNA和癌旁组织HBV DNA具有相关性(r=0.359、0.440,P<0.001),TNMⅠ期HCC患者HBcrAg与癌旁组织cccDNA和癌旁组织HBV DNA具有相关性(r=0.491、0.578,P<0.01),TNMⅡ期HCC患者HBcrAg与癌旁组织cccDNA和癌旁组织HBV DNA具有相关性(r=0.339、0.402,P<0.05),TNMⅢ期HCC患者HBcrAg与癌旁组织cccDNA和癌旁组织HBV DNA无相关性。结论 HBcrAg可以辅助区分发生HBe Ag血清学转换和未发生转换的患者;在乙型肝炎表面抗原(HBsAg)+HCC患者中,HBcrAg与癌旁组织HBV DNA及癌旁组织cccDNA具有一定的相关性,但其相关性未表现出比现有血清标志物(HBe Ag及HBV DNA)更好。Objective To assess the correlations of a newly-developed hepatitis B virus（HBV） infection marker,hepatitis B core-related antigen（HBcrAg）,with covalent closed circular DNA（cccDNA） and HBV DNA in tumor tissue and adjacent non-tumor tissue among hepatocellular carcinoma（HCC） patients. Methods A total of 95 HBV infection-related patients who received hepatic resection for HCC were enrolled. Serum HBcrAg were determined by chemiluminescence immunoassay. HBV DNA and cccDNA in tumor tissue and adjacent non-tumor tissue were quantified by polymerase chain reaction（PCR） fluorescent probe. Results The levels of HBcrAg in hepatitis B e antigen（HBeAg）＋/hepatitis B e antibody（HBeAb）-group,HBeAg＋/HBeAb＋group and HBeAg-/HBeAb＋group were （6.41±1.18）,（5.80±0.50） and（5.16±0.94）lgU/mL,respectively,and there was statistical significance among these 3 groups（P〈0.001）. There were correlations of HBcrAg with adjacent non-tumor tissue cccDNA and adjacent non-tumor tissue HBV DNA in HCC patients（r=0.359 and 0.440,P〈0.001）. In HCC patients of TNM stageⅠ,HBcrAg was associated with adjacent non-tumor tissue cccDNA and adjacent non-tumor tissue HBV DNA （r=0.491 and 0.578,P〈0.01）. In HCC patients with TNM stageⅡ,HBcrAg was correlated with adjacent non-tumor tissue cccDNA and adjacent non-tumor tissue HBV DNA（r=0.339 and 0.402,P〈0.05）. However, in HCC patients with TNM stageⅢ,no correlation of HBcrAg and adjacent non-tumor tissue cccDNA and adjacent non-tumor tissue HBV DNA. Conclusions HBcrAg can help with distinguishing HBeAg positive and negative HCC patients. Although HBcrAg has correlations with adjacent non-tumor tissue HBV DNA and cccDNA in hepatitis B surface antigen（HBsAg）＋HCC patients,it does not show better correlations than other serum markers such as HBeAg and HBV DNA.

关 键 词：乙型肝炎核心相关抗原 乙型肝炎E抗原 DNA 乙型肝炎病毒 原发性肝细胞癌 

分 类 号：R446.62[医药卫生—诊断学]