山东省柯萨奇病毒B1型VP1区全基因序列分析  

Phylogenetic Analysis of Complete VP1 Sequence of Coxsackievirus B1 in Shandong Province,China

作　　者：王海岩[1,2] 陶泽新[2] 李岩[2] 张文强[2] 刘晓林[2] 徐爱强[1,2] 

机构地区：[1]山东大学公共卫生学院,济南250014 [2]山东省疾病预防控制中心山东大学预防医学研究院山东省传染病预防控制重点实验室,济南250014

出　　处：《病毒学报》2016年第6期683-688,共6页Chinese Journal of Virology

基　　金：泰山学者工程专项经费资助(项目号:ts.201511105);山东省自然科学基金项目(项目号:ZR2012HM007);题目:柯萨奇病毒B病毒体外重组感染性克隆的构建及生物学特性的鉴定;国家自然科学基金项目(项目号:81302481);题目:环境污水中人类肠胃炎病毒的型别多样性;遗传进化规律及其与人群疾病发生的关系

摘　　要：为了解柯萨奇病毒B1型（Coxsackievirus B1,CV-B1）山东地方株的分子流行病学特征,本研究对1994年至2015年山东省急性弛缓性麻痹（Acute flaccid paralysis,AFP）监测系统、环境污水监测和无菌性脑膜炎病例标本中分离到的CV-B1病毒进行了VP1序列测定、系统发生学分析和同源性分析。共分离到CV-B1病毒53株,其中AFP监测、污水和及脑炎标本各分离到41株、4株和8株。基于VP1完整编码区序列的系统发生学分析显示CVB1山东株与国内其他分离株属于一个大的分支,该分支内无国外分离株,国外分离株构成了其他两个分支。山东株之间的VP1核苷酸同源性为84.4%-100.0%,与其他国家分离株的同源性为77.9%-85.0%。研究结果表明,中国CV-B1分离株与国外株相比有较大的遗传差异,需要加强相关手足口病病毒学监测,关注不同传播链的新的基因亚型的肠道病毒的输入。To investigate the molecular epidemiological characterization of coxsackieivirus B1（CV-B1）,we performed VP1 sequencing on all isolates from acute flaccid paralysis（AFP）surveillance,environmental surveillance and viral meningitis specimens from 1994 to 2015.A total of 53CV-B1 strains were isolated,among which 41 strains,4strains,and 8strains were obtained from AFP surveillance,environmental surveillance and meningitis specimens,respectively.Phylogenetic analysis based on VP1 entire coding region revealed that Shandong CV-B1 strains were segregated into a major cluster alongside with other domestic strains,with no foreign strains existing in this cluster.Foreign strains composed two other exclusive branches.Shandong strains had VP1 nucleotide similarities of 84.4%to 100.0% with each other,and 77.9%to 85.0% with foreign strains.The results presented here demonstrate Chinese CV-B1 strains have great genetic divergence with foreign strains.EV associated surveillance should be reinforced to monitor possible importation of different EV transmission lineages.

关 键 词：手足口病 柯萨奇病毒B1型 系统发生 肠道病毒 同源性 

分 类 号：R373.2[医药卫生—病原生物学]