江苏省2015年柯萨奇病毒A组16型VP1基因特征  

Genetic Characteristics of the VP1 Region of the Coxsackie A16 Virus from Jiangsu Province,China,in 2015

作　　者：黎俊宏[1] 嵇红[2] 樊欢[2] 李琼[1] 王凤鸣[1] 姚萍[1] 单军[2] 

机构地区：[1]常州市疾病预防控制中心检测检验中心,江苏常州213022 [2]江苏省疾病预防控制中心,江苏南京210009

出　　处：《病毒学报》2016年第6期689-693,共5页Chinese Journal of Virology

基　　金：国家自然科学基金青年基金(项目号:81402732);题目:重症手足口病后遗症队列随访及其病原演变的特征性研究

摘　　要：分析江苏省2015年柯萨奇病毒A组16型（CA16）分离株VP1基因特征。对江苏省2015年分离得到的20株CA16毒株进行VP1区基因扩增和扩增产物测序,并应用Mega 6.0和DNA Star软件对其构建基因进化树并分析核苷酸和氨基酸同源性。20株CA16分离株VP1核苷酸和氨基酸同源性分别为88.2%-100.0%和98.0%-100.0%;与A型国际原型株G10核苷酸和氨基酸同源性分别为75.3%-77.4%和90.6%-92.3%;与B1型代表株核苷酸和氨基酸同源性分别为88.3%-98.4%和96.3%-100.0%;与B2型代表株核苷酸和氨基酸同源性分别为88.4%-90.8%和96.6%-100.0%。20株CA16分离株基因亚型均为B1型,其中19株为B1b型,1株为B1a型,并有1个B1b传播链。江苏省2015年CA16分离株属于B1基因亚型,存在B1a和B1b两个进化分支共同进化和循环,其中以B1b为绝对优势流行株。The aim of this study was to analyze the genetic characteristics of the VP1 gene of coxsackievirus A16（CA16）strains isolated from Jiangsu Province,China,in 2015.The VP1 regions of 20CA16 virus strains from Jiangsu Province in 2015 were amplified,and the amplification products were sequenced.Mega 6.0and DNAStar software were applied to build the phylogenetic tree and analyze the homogeneity of nucleotides and amino acids.The sequence homologies of the nucleotides and amino acids of the VP1 gene were 88.2%-100.0%and 98.0%-100.0%among 20CA16 isolates,respectively.Comparison with the sequence of the prototype strain A-G10 showed 75.3%-77.4% homologies in nucleotide sequence and90.6%-92.3%in amino-acid sequences,respectively.Comparison with the sequence of the representative strain B1 showed 88.3%-98.4% homologies in nucleotide sequence and 96.3%-100.0%in amino-acid sequence,respectively.Comparison with the sequence of the representative strain B2 showed 88.4%-90.8% homologies in nucleotide sequence and 96.6%-100.0%in amino-acid sequence,respectively.Twenty CA16 isolates were subgenotype B1.One isolate was subgenotype B1 a,whereas the remainder of isolates was subgenotype B1 bamong 20CA16isolates.A subgenotype B1 btransmission chain was also noted.The CA16 strains isolated from Jiangsu Province in 2015 belonged to subgenotype B1.There were two evolutionary branches,whereby B1 aand B1bwere co-circulating and evolving together.The epidemic strain was subgenotype B1 b.

关 键 词：手足口病 柯萨奇病毒A组16型 VP1 B1 

分 类 号：R373.2[医药卫生—病原生物学]