甘肃省瓜州县一起由埃可病毒30引致的无菌性脑炎疫情病原学分析  

Pathogenic Analyses of an Outbreak of Viral Encephalitis Caused by ECHO30 in Guazhou of Gansu Province,China

作　　者：陈建华[1] 朱贞[2] 李煜山 武海卓 刘海霞[1] 王旭霞[1] 于德山[1] 

机构地区：[1]甘肃省疾病预防控制中心,兰州730000 [2]中国疾病预防控制中心病毒病预防控制所卫生部医学病毒和病毒病重点实验室,北京102206 [3]瓜州县疾病预防控制中心,酒泉市瓜州县736100

出　　处：《病毒学报》2016年第6期721-726,共6页Chinese Journal of Virology

基　　金：国家"十二五"艾滋病和病毒性肝炎等重大传染病防治科技重大专项(项目号:2012ZX10004-208);题目:甘肃及周边省区传染病病原谱流行规律研究;甘肃省手足口病病原变异变迁研究(项目号GWGL2014-75);题目:甘肃省手足口病病原变异变迁研究

摘　　要：对2015年6-8月发生在中国甘肃省瓜州县一起病毒性脑炎疫情进行病原学诊断及其分子特征研究。采集74例病例样本132份（脑脊液14份,咽拭子25份,血清66份以及粪便27份）。对血清与脑脊液标本经ELISA IgM检测初步排除乙脑病毒、单纯疱疹病毒、流行性腮腺炎病毒和腺病毒感染之后,采用实时荧光PCR法对所有标本进行人类肠道病毒核酸检测,阳性者使用HEp-2和RD细胞进行病毒分离,并对分离株进行全长VP1区核苷酸序列测定及其分子特征分析。结果发现：132份标本中人类肠道病毒核酸检测阳性72份,71份分子定型结果为埃克病毒30（E-30）;从29例病例的46份样本分离到E-30;甘肃瓜州E-30的全长VP1区核苷酸序列的同源性高达99.2%-100.0%,进化树图显示其与中国2011年以后的E-30分离株属同一个分支。E-30是引起本次甘肃省瓜州县局部地区病毒性脑炎暴发的病原,且属于中国正在流行的ECHO30进化分支中近期流行簇。We used molecular-biology methods to identify the pathogens that caused an outbreak of viral encephalitis in Guazhou（Gansu province,China）during June-August 2015.We also undertook molecular characterizations of these pathogens.A total of 132samples（14cerebrospinal fluid（CSF）samples;25throat swabs;66serum samples;27fecal samples）were collected from 74 patients during the outbreak of viral encephalitis.For CSF and serum samples,enzyme-linked immunosorbent assay immunoglobulin-M tests were undertaken to detect Japanese encephalitis viruses,enteroviruses,herpes simplex viruses,mumps viruses,and adenoviruses.Real-time polymerase chain reaction was done to detect enteroviruses（including coxsackievirus A16 and enterovirus 71）and the RNA of human adenoviruses.Then,viral isolation was carried out using HEp-2and RD cells,and the entire VP1 region of positive viral isolates was amplified and sequenced.Finally,molecular characterizations of these pathogens were completed.Seventytwo samples were identified as enteroviruses from 132 samples.Among them,71 were identified as echovirus（ECHO）30using enterovirus molecular typing.Japanese encephalitis viruses,herpes simplex viruses,mumps viruses,and adenoviruses were not detected.ECHO30 was isolated from 46 samples out of 29 patients.Similarities in nucleic acids among these ECHO30 isolates were 99.2%-100.0%.ECHO30 from Gansu province and other ECHO30 strains isolated in China since 2011 belonged to a same evolutionary branch.ECHO30 was the pathogen that caused the outbreak of viral encephalitis in Guazhou in 2015.ECHO30 from and Gansu province and ECHO30 isolated in China since 2011 belonged to the same evolutionary branch.

关 键 词：病毒性脑炎 埃可病毒30型(ECHO30) VP1区 序列分析 

分 类 号：R373.24[医药卫生—病原生物学] R373.31[医药卫生—基础医学]