雷公藤甲素对实验性大鼠结肠炎组织中细胞因子IFN-γ、IL-12表达的影响  

Effects of tripotlide on the expression of IFN-γ and IL-12 of intestinal mucosa in rats with colitis

作　　者：杨立[1] 肖明明[1] 李艳[1] 邢煜[1] 王岩[1] 姚远[1] 

机构地区：[1]辽宁省人民医院消化内一科,沈阳110016

出　　处：《实用药物与临床》2016年第11期1332-1336,共5页Practical Pharmacy and Clinical Remedies

基　　金：辽宁省自然科学基金课题(201102106)

摘　　要：目的探讨雷公藤甲素对TNBS诱导大鼠结肠炎的疗效及对炎性细胞因子IL-12和IFN-γ表达的影响。方法将18只成年SD大鼠随机分为对照组、模型组及雷公藤甲素组,观察大鼠的一般情况,比较结肠组织炎症程度。HE染色观察大鼠结肠组织形态学变化。用反转录聚合酶链反应(RT-PCR)和Western blot(WB)法检测大鼠结肠组织中IL-12、IFN-γ的表达水平。结果对照组大鼠结肠组织中IL-12和IFN-γ的表达水平最低,与对照组相比,模型组大鼠结肠组织中IL-12、IFN-γ表达显著增高(P<0.01),与模型组相比,雷公藤甲素组TL-12、IFN-γ表达明显降低(P<0.05)。雷公藤甲素治疗对大鼠结肠组织及大体变化都有一定程度的改善。结肠组织IFN-γ和IL-12的阳性细胞表达与结肠炎病情轻重呈正相关。结论雷公藤甲素能有效治疗TNBS诱导的大鼠结肠炎,其作用机制可能与雷公藤甲素下调大鼠结肠黏膜炎性细胞因子IL-12和IFN-γ的表达,抑制炎症的发生发展有关。Objective To evaluate the curative effect and regulation of tripotlide on the expression of intestinal mucosa IL-12 and IFN-γ in rats with colitis induced by trinitrobenzene sulfonic acid（TNBS）.Methods Eighteen adult Sprague-Dawley rats were averagely randomized into three groups：control group,model group,tripotlide treatment group.After treatments,the general status of all rats was observed;the histopathologic changes in the colon were examined;reverse transcription-polymerase chain reaction and Western blot techniques were used to detect the expression of cytokines IL-12 and IFN-γ.Results Compared with control group,the immunoreactive positive cells of IL-12 and IFN-γ increased significantly in model group.Compared with model group,the expression of 11-12 and IFN-γ in tripotlide treatment group decreased significantly（P〈0.05）.It indicated that tripotlide could decrease the expression of IL-12 and IFN-γ in the colonic mucous membrane.Conclusion Tripotlide can effectively treat TNBS-induced colitis in rats by down-regulating the expression of IL-12 and IFN-γ and relieving the inflammation.

关 键 词：雷公藤甲素 实验性结肠炎 IFN-Γ IL-12 

分 类 号：R285.5[医药卫生—中药学]