山西猪链球菌2型主要毒力相关基因的克隆及分析  

Cloning and Analysis on the Major Virulence-associated Gene in Shanxi Strain of Streptococcus Suis Serotype 2

作　　者：王红宝[1] 李红丽[1] 郭雪丽[1] 韩惠瑛[2] 杨晋青[1] 段亚娜[1] 王志俊[1] 吉涛[1] 

机构地区：[1]山西省农业科学院畜牧兽医研究所,山西太原030032 [2]山西省动物疫病预防控制中心,太原030027

出　　处：《养猪》2016年第6期107-110,共4页Swine Production

基　　金：山西省农业科学院科技攻关项目(2012ygg35)

摘　　要：为了解37株猪链球菌2型（S.suis 2）山西分离株的毒力基因型及毒力基因变异情况,通过PCR扩增S.suis 2毒力基因GDH、SLY、EPF、MRP,对这4种毒力基因的扩增片段进行测定,并与国内外其他分离株的基因进行比较。结果显示,GDH、SLY、EPF、MRP基因在37株S.suis 2中的检出率分别为86.5%、70.3%、56.8%、62.2%;37个受试菌株毒力基因型主要为GDH＋/SLY＋/EPF＋/MRP＋占51.35%,为优势基因型;S.suis 2山西分离株4种毒力基因与国内其他地区S.suis 2分离株的相应基因之间同源性均在99.1%以上,与国外S.suis 2分离株的相应序列同源性在89.9%-100%之间。To understand 37 strains of Streptococcus suis serotype 2（S.suis 2） from Shanxi isolates of virulence genes and virulence gene variants, by PCR amplification S. suis 2 virulence genes GDH, SLY, EPF, MRP, of the four kinds of virulence gene fragments amplified were determined, and comparing with other isolates genetic at home and abroad. Results show that the GDH, SLY, EPF, MRP gene in 37 strains S. suis 2 detection rate respectively in：86.5%, 70.3%, 56.8%, 62.2%; 37 participants strain virulence genes on GDH＋/SLY＋/EPF＋/MRP＋is the main advantage of accounted for 51.35% of participants strain genotype; S. suis 2 isolates in Shanxi four virulence genes and other domestic regions S. suis corresponding gene homology between two isolates were over99.1%, with foreign S. suis 2 isolates the corresponding sequence homology between 89.9%~100%.

关 键 词：山西 猪链球菌 毒力基因 克隆 分析 

分 类 号：S858.28[农业科学—临床兽医学]