黄芪药材主要成分的含量测定方法及高效液相指纹图谱研究  

Study on the main component determination method and HPLC fingerprint of Radix Astragali

作　　者：张妍[1,2] 董琳[1] 雍婧姣 毛福英[1] 付雪艳[1] 

机构地区：[1]宁夏医科大学宁夏回药现代化工程技术研究中心,宁夏银川750001 [2]宁夏医科大学宁夏回医药现代化省部共建教育部重点实验室,宁夏银川750001

出　　处：《时珍国医国药》2016年第11期2610-2613,共4页Lishizhen Medicine and Materia Medica Research

基　　金：宁夏自然科学基金(No.NZ14060)

摘　　要：目的 建立高效液相色谱法测定黄芪药材中四种黄酮类成分的含量测定方法与黄芪药材指纹图谱。方法 采用Agilent SB-C18色谱柱（250 mm×4.6 mm,5μm）;乙腈-0.3%甲酸水溶液梯度洗脱为流动相;流速1ml·min^-1;柱温为35℃;检测波长254 nm。结果 四种黄酮的线性范围、相关系数良好（r=0.9996～0.9999）,加样回收率试验的RSD均小于1.85%;经过主成分分析,为黄芪药材进行了综合排名;建立了黄芪药材的HPLC指纹图谱,指定了20个共有峰,18批样品相似度均在0.9以上;经过聚类分析,将21批黄芪药材分为三类。结论 该方法简便可行、灵敏度高,可用于黄芪药材的质量控制。Objective To establish an HPLC method for determination of four flavonoids and fingerprint of Radix Astragali. Methods Agilent SB - C18 column （250mm×4.6 mm,5 μm）was used. The mobile phase consisted of acetonitrile -0.3 % formic acid with a gradient elution, the flow rate was 1 mL · min^-1. The column temperature was set at 35℃, and the detecting wave length was at 254 nm. Results The four flavonoids showed good linear relationship （ r = 0. 9996 - 0. 9999）, the average recoveries with RSD were less than 1.85 % ;principal components analysis of Radix Astragali was ranked;the similarity was more than 0.9 among 20 labeled mutual peaks by determination of 18 batches of Radix Astragali, cluster analysis of 21 batches of Radix Astragali was divided into three categories. Conclusion This method is convenient, high sensitivity and can be used in the quality control of Radix Astragali.

关 键 词：黄芪 黄酮类成分 含量测定 主成分分析 指纹图谱 聚类分析 

分 类 号：R284.1[医药卫生—中药学]