放线菌SSPRC-1活性代谢产物的分离鉴定  

Isolation and identification of bioactive metabolites from Actinomycetes SSPRC-1

作　　者：王雪啸 宗蜜蜜 徐文平[1] 程亮[1] 陶黎明[1] Wang Xue-xiao Zong Mi-mi Xu Wen-ping Cheng Liang Tao Li-ming(Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science ＆ Technology, Shanghai 20023)

机构地区：[1]华东理工大学药学院,上海市化学生物学(芳香杂环)重点实验室,上海200237

出　　处：《中国抗生素杂志》2016年第12期898-901,共4页Chinese Journal of Antibiotics

基　　金：“十二五”国家科技支撑项目绿色生态农药的研发与产业化(No.2011BAE06B04)

摘　　要：目的对土壤放线菌SSPRC-1次级代谢产物中杀虫和抑菌活性物质进行研究。方法对发酵液通过正丁醇萃取、硅胶柱色谱、HPLC等手段进行分离纯化,利用质谱、核磁共振谱等现代波谱分析方法鉴定活性化合物结构。结果从放线菌SSPRC-1发酵液中分离得到3个活性化合物SSPRC-1-1、SSPRC-1-2和SSPRC-1-3,确定SSPRC-1-1分子式为C15H10O4,是异黄酮类物质,在天然产物中未曾发现,仅有文献报道可通过化学合成得到,暂命名为isoflavones H;SSPRC-1-2分子式为C15H10O5,与异黄酮类物质三羟基异黄酮genistein相同;SSPRC-1-3分子式为C25H37NO4,与杀粉蝶毒素A结构相同。结论化合物SSPRC-1-1和SSPRC-1-2对Pyricularia oryzae的MIC分别为125和24μg/m L,化合物SSPRC-1-3对黏虫具有较好的毒杀作用。Objective To investigate the activities of the secondary metabolites against Pyricularia oryzae and Mythimna separate （Walker） from the strain Streptomyces SSPRC-1. Methods By bioassay-guided isolation and purification, the compounds were isolated and purified from fermentation with n-butyl, silica gel column chromatography and HPLC, and their structures were elucidated with the help of the spectra data analysis of UV, ESI- MS and NMR. Results Three compounds were isolated from the fermentation broth of Streptomyces SSPRC-1 and named as SSPRC-1-1, SSPRC-1-2 and SSPRC-1-3. The molecular formula of the compound SSPRC-1-1 was confirmed as C15H1004, which was identified as a new natural compound named isoflavone H. The molecular formulas of the compound SSPRC-1-2 and SSPRC-1-3 were confirmed as C15H1005 and C25H37NO4, the same as the known compound genistein and piericidin, respectively. Conclusion The MIC of two compounds SSPRC-1-1 and SSPRC-1-2 against P. oryzae were 125 and 24μg/mL. Compound SSPRC-1-3 showed good bioactive against Mythimna separate.

关 键 词：放线菌 分离纯化 结构鉴定 生物活性 

分 类 号：Q939[生物学—微生物学]