亲水作用色谱法测定纽莫康定B0及其杂质的研究  

Research on the determination of the contents of pneumocandins B_0 and related substance by hydrophilic interaction liquid chromatography

作　　者：杨渊[1] 孟慧云[1] 王欣荣[1] 翟龙飞[1] 褚以文[1] Yang Yuan Meng Hui-yun Wang Xin-rong Zhai Long-fei Chu Yi-wen(Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052)

机构地区：[1]抗生素研究与再评价四川省重点实验室,四川抗菌素工业研究所,成都大学,成都610052

出　　处：《中国抗生素杂志》2016年第12期927-930,共4页Chinese Journal of Antibiotics

摘　　要：目的建立同时检测纽莫康定发酵液中纽莫康定A0、Bo和C0的含量的HILIC方法a方法采用Xlon色谱柱（4．6mm×250mm，10μm）进行分离，以乙腈和水（87：13）为流动相；流速为2．0mL／min;检测波长：210rim；柱温：30℃进样量10此。结果纽莫康定A0、B0和c0三者质量浓度与峰面积分别在0．23-150μg／mL、0．12～100μg／mL、0．34～70μg／mL范围内线性关系良好，尺。均大于0．9993，平均加样回收率分别为96．73％（RSD，1．52％）、99．62％（RSD，1．77％）、102．35％（RSD，2．08％）。结论HILIC法同时检测纽莫康定A0、Bo和co含量的方法简单可行、精密度高、重复性好，为工业生产中分离纯化及质量控制提供依据。Objective To establish ahydrophilic interaction liquid chromatography （HILIC） method for the simultaneous determination of the contents of pneumocandins A0, B0 and CO in fermentation broth. Methods The separation was performed on an Xlon chromatographic column （4.6mmx250mm, 10μm） with acetonitrile-H20 （87：13） as the mobile phase at the flow rate of 2.0mL/min. The detection UV wavelength was at 210 nm. The column temperature was 30℃. The injection volume was 10pL. Results The linearities ranged in 0.23-150μg/mL for pneumocandin A0, 0.12-100μg/mL for pneumocandin B0, and 0.34-70μg/mL for penumocandin C0, respectively. Recoveries of pneumocandins in A0, B0 and CO were 96.73% with RSD of 1.52%, 99.62% with RSD of 1.77%, 102.35% with RSD of 2.08%, respectively. Conclusion The method is simple, accurate and highly reproducible, which provides the basis for the separation, purification and quality control in the industrial production.

关 键 词：纽莫康定 亲水作用色谱 含量测定 

分 类 号：R917[医药卫生—药物分析学]