建立大鼠骨性关节炎模型及补体复合物表达研究  

Increased expression of complement complex in cartilage of osteoarthritis

作　　者：张可可[1] 毕意辉 陈学周[1] 董洲[1] 张明凯[1] 尤涛[1] 

机构地区：[1]安徽医科大学第一附属医院骨科,合肥230022

出　　处：《安徽医科大学学报》2016年第12期1785-1790,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81171797);湖北省自然科学基金(编号:2011CHB022);中国博士后科学基金(编号:9021346201)

摘　　要：目的通过建立大鼠骨性关节炎(OA)模型探讨补体复合物(MAC)在软骨及滑膜中的表达。方法将30只SD大鼠随机分为对照组、4周组、8周组、12周组,每组10只。手术组取大鼠后肢一侧膝关节行前交叉韧带切断加内侧半月板切除术制造OA模型,手术后4周、8周及12周取两侧膝关节软骨行大体观察、HE染色、番红O-固绿染色、甲苯胺蓝染色估计软骨退变程度以及进行组织病理学的Mankin评分,免疫组化及Western blot法检测MAC在软骨中沉积及表达情况。结果与对照组比较,手术组大鼠膝关节软骨4周、8周及12周后呈现软骨粗糙、糜烂、裂隙形成、缺损及骨赘形成等早中晚期OA表现。各组间Mankin计分差异均有统计学意义(P<0.01),损伤逐渐加重。各组间滑膜评分差异有统计学意义(P<0.01),炎症逐渐降低。免疫组化结果显示软骨细胞膜周围出现环形MAC沉积,滑膜中没有MAC。Western blot检测显示实验组有MAC条带,对照组没有。结论 MAC在OA大鼠软骨中表达增多,补体系统可能作为诊治OA的新方向。Objective To investigate the role of MAC in cartilage and synovial membrane by establishing the model of rat osteoarthritis. Methods 30 SD rats were randomly divided into control group,4-week group,8-week group and 12-week group. 10 rats were in each group. One of the operation group rats hind knee joints suffered anterior cruciate ligament transection and medial meniscus resection to induce a model of OA,4 weeks,8 weeks and 12 weeks after surgery,both sides of the knee joint cartilages were removed for gross observation,hematoxylin and eosin（ HE） staining and safranin O-fast green double dyeing,toluidine blue staining to assess histopathological injures. Then immunohistochemistry and Western blot were used to detect the deposition and expression of MAC in cartilage. Results Compared with the control group,knee cartilages of operation groups after 4 weeks,8 weeks and 12 weeks were rough,contained erosion,defects,cracks and osteophyte formation from the early to the late stage of OA. There were statistically significant differences in Mankin score between the random two groups（ P〈0. 01）,and damage gradually increased. There was statistical significance in synovium score among the groups（ P〈0. 01）,and the inflammation gradually decreased. MAC deposited around the cartilage cell membrane in immunohistochemistry. There was no MAC in the synovial membrane basically. The results of Western blot showed that there was a MAC band in the experimental group,while the control group not. Conclusion MAC is expressed in cartilage of rats with osteoarthritis,and the complement system may be a new direction for the diagnosis and treatment of osteoarthritis.

关 键 词：骨性关节炎 补体 补体复合物 软骨 滑膜 

分 类 号：R684.3[医药卫生—骨科学]