多种肠道病毒引起手足口病细胞免疫功能比较  

Cellular immunity function comparison of hand foot and mouth disease caused by multiple intestinal viruses

作　　者：王宇婧[1] 程邦宁[1] 

机构地区：[1]安徽医科大学儿科临床学院(安徽省儿童医院)检验科,合肥230051

出　　处：《安徽医科大学学报》2016年第12期1836-1838,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省卫生和计划生育委员会科研计划项目(编号:14FR017)

摘　　要：目的探讨肠道病毒71型(EV71)、柯萨奇病毒A16型(CA16)、其他肠道病毒引起的手足口病(HFMD)患儿细胞免疫功能的不同。方法收集HFMD患儿咽拭子或肛拭子标本,进行EV71、CA16、EV核酸检测,根据荧光RT-PCR法检测结果,分为EV71阳性组、CA16阳性组、通用EV阳性组。应用流式细胞仪检测患儿外周血淋巴细胞亚群CD3+T、CD4^+T、CD8+T、NK、B细胞百分率。结果 EV71阳性组患儿CD3+T、CD4^+T细胞百分率明显低于CA16阳性组和EV阳性组患儿(P<0.05),CA16阳性组和EV阳性组患儿CD3+T、CD4^+T细胞百分率差异无统计学意义;3组患儿CD4^+T/CD8+T、CD8+T细胞百分率差异无统计学意义;EV71阳性组患儿NK细胞、B细胞百分率明显高于CA16阳性组和EV阳性组患儿(P<0.05),CA16阳性组和EV阳性组患儿NK细胞、B细胞百分率差异无统计学意义。结论EV71较CA16以及其他肠道病毒引起的HFMD患儿机体细胞免疫功能紊乱加重。Objective To investigate the difference of cellular immunity in children with HFMD caused by multiple viruses of EV71,CA16 and other intestinal viruses. Methods Collected throat swab or anal swab specimens from children with HFMD,and detected RNA of EV71,CA16 and EV. Patients were divided into three groups,including EV71 positive group,CA16 positive group and EV positive group according to the results of RT-PCR detection. Detected the percentage of lymphocyte subsets CD3＋T,CD4^＋T,CD8＋T,NK and B cell of peripheral blood by flow cytometry. Results The CD3＋T,CD4^＋T cell percentage of EV71 positive group was significantly lower than other two groups（ P〈0. 05）,the CD3＋T,CD4^＋T cell percentage between CA16 positive group and EV positive group was not obviously different. The ratio of CD4^＋T/CD8＋T and CD8＋T cell percentage was not obviously different among these three groups. Meanwhile,the percentage of NK,B cell of EV71 positive group was significantly higher than other two groups（ P〈0. 05）,but there was no significant difference of NK,B cell percentage between CA16 positive group and EV positive group. Conclusion EV71 can cause severer dysfunction of cellular immunity rather than CA16 or other intestinal viruses.

关 键 词：肠道病毒 手足口病 细胞免疫 

分 类 号：R373.2[医药卫生—病原生物学] R725.125[医药卫生—基础医学]