TNF-α对大鼠舌黏膜创伤愈合过程中上皮间质转化的影响  

Effect of TNF-α on EMT and wound healing during rats' tongue mucosa injury

作　　者：王飞虎[1] 孙应明[2] 孔苗苗[3] 王飞[1] 杨聪翀[4] 刘来奎[4] 

机构地区：[1]安徽医科大学无锡临床学院口腔科,无锡214044 [2]解放军101医院口腔科,无锡214044 [3]合肥市口腔医院正畸科,合肥230001 [4]南京医科大学附属口腔医院基础教研室,南京210029

出　　处：《安徽医科大学学报》2016年第12期1863-1866,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81272968);南京军区医药卫生科研基金(编号:ZD05)

摘　　要：取60只8周龄Wistar大鼠,随机分为TNF-α+凝胶实验组和空白凝胶对照组,于其舌背部黏膜处作一圆形缺损模型;每组大鼠分别于0、24、48、72、96 h处死,取标本冰冻切片。通过免疫组织荧光法检测与上皮间质转化(EMT)相关蛋白E-钙黏蛋白(E-cad)、成纤维细胞特异性蛋白-1(FSP1)和波形蛋白(Vimentin)在大鼠舌黏膜创伤愈合过程中的表达。结果显示两组大鼠创伤部位上皮细胞均有表达FSP1和Vimentin,实验组发生改变的细胞数目明显较对照组多,差异有统计学意义(P<0.05)。60 8-week-old Wistar rats were randomly divided into TNF-α ＋ Gel experimental group and the Gel control group. A 3 mm-diameter circular defects were made on the tongue mucosa of the rats. The rats were killed at 0,24,48,72 and 96 hour after operations. Immunohistochemical fluorescence was used to detect the expression of EMT related protein E-cadherin（ E-cad）,fibroblast specific proteins（ FSP1） and Vimentin in tongue mucosa during wound healing. The number of cells which expressed Vimentin and FSP1 in the basal layer of wound edge zone in TNF-α group was more than that in the control group（ P〈0. 05）.

关 键 词：创伤愈合 舌黏膜 上皮间质转化 TNF-Α 

分 类 号：R782.4[医药卫生—口腔医学]