南京莫愁湖与紫霞湖浮游细菌群落结构的季节性变化及其与环境因子的关系  

Seasonal Variation of Bacterioplankton Community Structure and Its Relationship with Environmental Factors of Mochou Lake and Zixia Lake in Nanjing

作　　者：曹新益 徐慧敏[1,2] 王司辰 黄睿[1,2] 沈烽[1,2] 何肖微 余钟波[1,2] 赵大勇[1,2] 

机构地区：[1]河海大学水文水资源与水利工程科学国家重点实验室,江苏南京210098 [2]河海大学水文水资源学院,江苏南京210098

出　　处：《化学与生物工程》2016年第12期19-26,30,共9页Chemistry & Bioengineering

基　　金：国家自然科学基金资助项目(41371098);水文水资源与水利工程科学国家重点实验室专项研究经费资助项目(20145027312;20155019012);中央高校基本科研业务费资助项目(2015B14214)

摘　　要：应用16SrRNA基因末端限制性片段长度多态性(T-RFLP)技术和多元统计方法研究了南京莫愁湖和紫霞湖浮游细菌群落结构的季节性变化及其与环境因子的关系。结果表明,莫愁湖和紫霞湖的浮游细菌群落alpha多样性指数在夏季和秋季时最高,在春季和冬季时最低;紫霞湖冬季浮游细菌群落alpha多样性指数显著大于莫愁湖。非度量多维尺度(NMDS)分析结果显示,莫愁湖和紫霞湖浮游细菌群落结构存在明显的季节性变化。Mantel检验、典范对应分析(CCA)表明,影响莫愁湖和紫霞湖浮游细菌群落结构最显著的环境因子是温度和pH值,营养物对莫愁湖和紫霞湖浮游细菌群落结构的影响程度取决于湖泊的营养水平。为进一步研究不同程度富营养化水体中的浮游细菌群落结构差异提供了帮助。Seasonal variation of bacterioplankton community structure and its relationship with environmental factors of Mochou Lake and Zixia Lake in Nanjing were studied by 16S rRNA terminal restriction fragment length polymorphism（T-RFLP） technology and multivariate statistical methods. The results showed that,alpha -diversity indexes of bacterioplankton community from Mochou Lake and Zixia Lake were the highest in sum- mer and autumn,and the lowest in spring and winter. The alpha-diversity index of bacterioplankton community from Zixia Lake was significantly greater than that from Mochou Lake in winter. Non-metric multidimensional scaling（NMDS） analysis showed that, bacterioplankton community structure of Mochou Lake and Zixia Lake had a seasonal variation. Mantel test,canonical correlation analysis（CCA） showed the most significant environ- mental factors which affected the bacterioplankton community structure of Mochou Lake and Zixia Lake were temperature and pH value. Effects of nutrient on bacterioplankton community structure of Mochou Lake and Zixia Lake were depended on the nutrient levels in lake. Overall, this study provides a help to research difference of hacterioplankton community structure in various eutrophic waters.

关 键 词：莫愁湖 紫霞湖 浮游细菌 群落结构 季节性变化 环境因子 多元统计 末端限制性片段长度多态性技术 

分 类 号：Q178.1[生物学—水生生物学] X172[生物学—普通生物学]