雷公藤内生真菌Pestalotiopsis disseminata次级代谢产物研究  

Secondary Metabolites of Endophytic Fungus Pestalotiopsis disseminata Isolated fromTripterygium wilfordii Hook.f.

作　　者：李博[1] 王宇[1] 潘华奇[2] 梁洪玥 赵楠[1] 陈再兴[4] 刘涛[1] 

机构地区：[1]中国医科大学药学院天然药物化学教研室,辽宁沈阳110122 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [3]锦州医科大学药学院,辽宁锦州121000 [4]中国医科大学药学院中心实验室,辽宁沈阳110122

出　　处：《化学与生物工程》2016年第12期27-30,共4页Chemistry & Bioengineering

基　　金：国家自然科学基金资助项目(81302672);辽宁省教育厅科学研究一般项目(L2013315)

摘　　要：采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱及PHPLC等手段对雷公藤(Tripterygium wilfordii Hook.f.)内生真菌广布拟盘多毛孢(Pestalotiopsis disseminata)的发酵次级代谢产物进行分离纯化,通过理化性质及波谱学数据分析对化合物结构进行鉴定。结果表明,从内生真菌Pestalotiopsis disseminata的次级代谢产物中共分离并鉴定了7个单体化合物,分别为7-羟基-5-甲氧基-4,6-二甲基苯酞(Ⅰ)、腺苷(Ⅱ)、尿苷(Ⅲ)、邻苯二甲酸二正丁酯(Ⅳ)、邻苯二甲酸二异丁酯(Ⅴ)、1,1′-二(1,1′-羧基乙基)醚(Ⅵ)、赤藓醇(Ⅶ),其中化合物Ⅱ、Ⅲ、Ⅵ、Ⅶ为首次从该属真菌中分离得到,化合物Ⅰ、Ⅴ为首次从该种真菌中分离得到。The secondary metabolites of Pestalotiopsis disseminata, a endophytic fungus isolated from Tripterygiurn wilfordii Hook. f. ,were isolated and purified through silica gel and Sephadex LH-20 gel column chromatography as well as PHPLC method,and the structures of obtained compounds were identified by physi- cochemical properties and spectral data. Results showed that, seven compounds which isolated from the seconda- ry metabolites of Pestalotiopsis disseminata were identified as 7-hydroxy-5-methoxy-4,6-dimethylphthalide （ Ⅰ ） ,adenosine（ Ⅱ ）, uridine（ Ⅲ ）, dibutyl phthalate（ Ⅳ ）, diisobutyl phthalate（Ⅴ ）, 1,1Cbis（1, l＇-carboxyethyl） ether（gl）, erthyritol（ VH ）, respectively. Compounds Ⅱ ,Ⅲ, Ⅳ and Ⅴ were firstly obtained from Pestalotiopsis genus,and compounds Ⅰ and Ⅴ were firstly found from fungus Pestalotiopsis disserninata.

关 键 词：雷公藤 内生真菌 广布拟盘多毛孢 次级代谢产物 

分 类 号：R284.1[医药卫生—中药学]