中华绒螯蟹蜕壳生长及其与相关基因表达的关联分析  

Observation on Individual Molting, Growth and Association Analysis with Relative Gene Expression in Chinese Mitten Crab (Eriocheir sinensis)

作　　者：陈娇[1] 岳武成 陈晓雯[1] 慈元吉 黄姝[1] 王军[1] 王成辉[1] 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306

出　　处：《动物学杂志》2016年第6期1059-1070,共12页Chinese Journal of Zoology

基　　金：上海市中华绒螯蟹产业技术体系项目[沪农科(产)字2010-4号];上海市科委崇明科技专项项目(No.13391912102);上海市工程技术中心建设项目(No.03DZ2251800)

摘　　要：蜕壳是甲壳动物常见的生长发育现象,但对调控蜕壳与生长的内在机制尚缺乏足够了解。本研究在室内条件下,对中华绒螯蟹(Eriocheir sinensis)一个蜕壳周期内的个体蜕壳与生长现象进行了连续观察,分析了2个蜕壳相关基因,即蜕皮激素受体基因(Ec R)和维甲类X受体基因(RXR),以及1个生长相关基因肌肉生长抑制素基因(MSTN)的表达及其与生长性状的相关性。结果发现,中华绒螯蟹在蜕壳后会出现一个跳跃式生长期,之后进入了一个缓慢持续上升过程,当营养物质积累到一定程度(肥满度达60%左右时)时启动下一次蜕壳;MSTN基因的表达与壳长(r=﹣0.450,P<0.05)、壳宽(r=﹣0.410,P<0.05)增长率呈显著负相关,而与肥满度呈显著正相关(r=0.450,P<0.05),Ec R和RXR基因的表达与体重、壳长和壳宽的增长率均没有显著相关性;相对来说,MSTN在蜕壳后的表达量越高,则增重率越小;而Ec R和RXR在蜕壳后表达量越高,其增重率越大。本研究结果表明,中华绒螯蟹在蜕壳后其生长具有一定的规律性,肥满度可以作为衡量中华绒螯蟹体内营养积累启动蜕壳的指标,Ec R、RXR及MSTN基因表达与生长表型具有一定的相关性。Molting is an essential biological process for the growth and development of crustaceans, however, the molecular mechanism of molting on growth regulation is little known. In this study, molting and growth performance were individually observed under the indoor laboratory condition, and gene expression level of two molting-related genes （ecdysteroid receptor gene, EcR; retinoid X receptor, RXR）, and one growth related gene （myostatin, MSTN） were investigated using quantitative RT-PCR method （Table 1 and Fig. 4）. A total of 60 juvenile crabs in each male and female groups were reared in 120 water closets of 60 L with 1 individual in each closet for two molting times. The growth traits （body weight, carapace length, carapace width, and merus length of second foot） were measured for each crab at the initial stocking stage, on the fifth day after the first molting and the second day after the second molting. Meantime, muscle and hepatoparncreas tissues were immediately collected and stored in ﹣80℃ freezer after growth traits measurements at the second molting for further gene expression analysis. The correlation analysis was conducted between the expression level of the three genes （EcR, RXR and MSTN） and growth traits using SPSS software. A sharp gaining of body weight was found after molting, followed by a slow and continuous increment in post-molting stage （Fig. 1, 2）. Condition factor that could be used as an index indicating the next molting was initiated when it accumulated over 60% （Fig. 3）. There was no significant correlation between the conditional factor and morphological character （Table 2）. Significant negative correlation was found between MSTN expression level and the growth rate of carapace length （r =﹣0.450, P 〈 0.05） and carapace width （r =﹣0.410, P 〈 0.05）, but significantly positive correlation was detected between MSTN expression level and condition factor （r = 0.450, P 〈 0.05） （Table 3）. There was no significant correlation between th

关 键 词：中华绒螯蟹 蜕壳 生长性状 基因表达 

分 类 号：S917.4[农业科学—水产科学]