急性肺血栓栓塞症犬模型的建立  

Establishment of canine acute pulmonary thromboembolism model

作　　者：王洋[1] 杨媛华[2] 李洪霞[1] 方向群[1] 王辰[3] 

机构地区：[1]解放军总医院南楼呼吸科,北京f100853 [2]首都医科大学附属北京朝阳医院北京呼吸疾病研究所 [3]中日友好医院

出　　处：《中华老年心脑血管病杂志》2016年第12期1247-1250,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家科技支撑计划(2006BAI01A06;2011BAI11B17);北京市科技计划项目(H030930020430);解放军总医院科技创新苗圃基金(14KMM18);总后保健专项科研课题(13BJZ44)

摘　　要：目的通过股静脉注入自体血栓,建立急性肺血栓栓塞症犬模型。方法选择杂种犬22只,由股静脉注入条形自体血栓,制成肺血栓栓塞症模型。监测注栓前、注栓后、造模后2h、造模后3h的血流动力学,检测平均肺动脉压(mPAP)、肺血管阻力(PVR)、心输出量(CO);行肺动脉造影,并使用改良Miller指数(MMI)进行量化评分。结果注栓后mPAP、PVR较注栓前明显升高[(26.01±6.34)mm Hg vs(15.62±2.30)mm Hg,1mm Hg=0.133kPa;(695.30±238.68)dyn/(s·cm5)vs(281.24±93.39)dyn/(s·cm5),1dyn=10-5 N,P<0.01)],CO较注栓前明显降低[(2.25±0.38)L/min vs(2.96±0.97)L/min,P<0.01)]。造模后2h和3h血流动力学指标与注栓后比较,差异无统计学意义(P>0.05)。注栓后MMI升至(17.23±6.47)分,与mPAP呈正相关(r=0.723,P<0.01)。结论 40ml自体血体外形成血凝块经股静脉注入,能模拟急性肺血栓栓塞症血流动力学、影像学和病理学变化,是良好的急性肺血栓栓塞症犬模型。Objective To establish the canine acute pulmonary thromboembolism （PTE） model by injecting autologou：s blood clots into the femoral vein. Methods Twenty-two canines were includ- ed in this study. Tihe canine acute PTE model was established by injecting autologous blood clots into the femoral vein. The hernodynamics, mPAP, PVR and CO were measured before and after in- jecting autologous blood clots,2 and 3 h after the model was established. The animals underwent pulmonary angiography and were scored according to the MMI. Results The mPAP and PVR were significantly higher whereas the CO was significantly lower after injecting autologous blood clots than before injecting autologous blood clots （26.01±6.34 mm Hg vs 15.62±2.30 mm Hg, 695.30±238. 68 dyn/s ; cm^5 vs 281. 24±93.39 dyn/s·cm^5 ,P〈0. 01;2. 25±0. 38 L/min vs 2.96±0.97 L/min,P〈0.01）. No significant difference was found in hymodynamics 2 and 3 h after injecting autologous blood clots. The MMI increased to 17.23±6.47 after injecting autologous blood clots and was positively related with the mPAP （r=0. 723,P〈0.01）. Conclusion Injection of 40 ml autologous blood clots into the femoral vein can show the changes in hemodynamics,angiogra-phy and pathology of acute PTE,and can thus establish the canine acute PTE model.

关 键 词：肺栓塞 血流动力学 肺动脉 血管造影术 模型 动物 

分 类 号：R563.5[医药卫生—呼吸系统]