茜素红与钙黄绿素标记不同规格黑鲷幼鱼的比较研究  

Comparative study on immersion marking with alizarin red S and calcein for black porgy Acanthopagrus schlegelii of different sizes

作　　者：刘岩[1] 孙典荣[1] 耿倩[1] 杨长平[1] 赵静 段妍[3] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东广州510300 [2]大连市水产研究所,辽宁大连116013 [3]辽宁省海洋水产科学研究院,辽宁省海洋生物资源与生态学重点实验室,辽宁大连116023

出　　处：《南方水产科学》2016年第6期17-24,共8页South China Fisheries Science

基　　金：公益性行业(农业)科研专项经费项目(201303048);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2015TS07);农业部财政项目“大亚湾增殖放流效果评估和跟踪监测”(2130135020)

摘　　要：采用茜素红(ARS)和钙黄绿素(CAL)以浸染方式对大、小2种规格的黑鲷(Acanthopagrus schlegelii)幼鱼进行荧光标记技术研究。急性染色实验结果显示,2种染料对2种规格黑鲷幼鱼成活率无显著影响;后续养殖实验结果表明,两者对2种规格黑鲷幼鱼生长率亦无显著影响。ARS和CAL对黑鲷幼鱼硬组织的标记效果随着染料浓度的增加而提高,ARS标记效果优于CAL。比较2种规格的黑鲷幼鱼荧光标记结果,ARS未见显著性差异,CAL小规格标记效果优于大规格。比较各取样组织标记效果,ARS对鳍棘标记取得了最好的标记效果,鳞片和鳍条次之,耳石标记效果最差;CAL对鳍棘、鳍条、鳞片的标记效果相似或等级相同,耳石次之。ARS标志技术更具应用和研究价值。We used alizarin red S （ARS） and ealeein （CAL） to dip black porgy （Acanthopagrus schlegelii） of two sizes by fluorescent markers technique. Acute staining results show that ARS and CAL had no significant effects on the survival rate of the juveniles of both sizes. Subsequent breeding test shows no significant effects on juveniles of both sizes. The markers effects of ARS and CAL on hard tis- sues of the juveniles increased with increasing dye concentration, but the markers effects of ARS were better than those of CAL. No significant difference was observed for ARS, but the marker effect of CAL of small size was better than that of big size. The marker effect of ARS of fin spines was the best, followed by scales and fins, and the worst was that of otolith. CAL marker effects were at the same level for spines, fins and scales, followed by otolith. ARS marking technology has more application and research value.

关 键 词：黑鲷 茜素红 钙黄绿素 荧光标记技术 硬组织 

分 类 号：S931.9[农业科学—渔业资源]