表面活性剂-超声协同提取山香圆叶总黄酮工艺的优化  

Optimization of surfactant assisted ultrasonic extraction for total flavonoids from Turpinia arguta leaves

作　　者：罗开沛 李小芳[1] 林浩[1] 罗佳[1] 杨露[1] 刘海霞[1] 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中成药》2016年第12期2585-2589,共5页Chinese Traditional Patent Medicine

基　　金：四川省教育厅重点项目(15ZA0094);四川省科技厅“苗子工程”重点项目(2016RZ0037)

摘　　要：目的优化表面活性剂-超声协同提取山香圆Turpinia arguta Seem.叶总黄酮工艺。方法在单因素试验基础上,以十二烷基硫酸钠(SDS)用量、液料比、乙醇体积分数为影响因素,总黄酮提取率为评价指标,Box-Behnken设计优化提取工艺。结果最佳条件为十二烷基硫酸钠用量0.64%,液料比25∶1,乙醇体积分数50%,超声温度60℃,超声时间40 min,总黄酮提取率为3.48%。结论该方法简单、节能、省时,可用于山香圆叶总黄酮的提取。AIM To optimize the surfactant assisted ultrasonic extraction process for total flavonoids from Turpinia arguta Seem. leaves. METHODS Based on single factor test,sodium dodecyl sulfate（ SDS） amount,liquid-solid ratio and ethanol concentration were determined to be the influencing factors,and Box-Behnken design was applied to optimizing the extraction with the extraction rate of total flavonoids as an evaluation tool. RESULTS The optimal conditions were determined to be 0. 64% for sodium dodecyl sulfate consumption,25 ∶ 1 for liquidsolid ratio,50% for ethanol concentration,60℃ for ultrasonic temperature,and 40 min for ultrasonic time at3. 48% total flavonoids extraction rate. CONCLUSION This energy and time saving,and yet simple method can be used for the extraction of total flavonoids from T. arguta leaves.

关 键 词：山香圆 叶 总黄酮 表面活性剂-超声协同提取 BOX-BEHNKEN设计 

分 类 号：R284.2[医药卫生—中药学]