零售肉品中产气荚膜梭菌的检测与定型:以陕西关中地区为例  

Detection and typing of Clostridium perfringens from retail meat in Guanzhong,Shaanxi

作　　者：姜艳芬[1] 王清爱 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]陕西镇巴县畜牧兽医工作站,陕西镇巴723600

出　　处：《西北农林科技大学学报（自然科学版）》2016年第11期55-60,69,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：西北农林科技大学基本科研业务费创新专项(QN2012019)

摘　　要：【目的】检测陕西关中地区肉品中的产气荚膜梭菌,并进行血清型和产气荚膜梭菌肠毒素(CPE)基因型鉴定,初步掌握该地区肉品中产气荚膜梭菌的污染状况。【方法】从陕西西安、杨凌、武功等地的超市、农贸市场、肉食品小摊随机采集新鲜生熟鸡、猪肉及其制品,共计314份,经细菌分离纯化、染色镜检、生化试验和单重PCR检测确定分离菌株为产气荚膜梭菌,应用多重PCR检测分离菌株的血清型并进行cpe毒素基因检测。【结果】样品中产气荚膜梭菌检出率为45.86%,且均为A型cpe产气荚膜梭菌,其中熟肉制品、生鲜肉检出率分别为50.91%和43.14%;鸡肉、猪肉检出率分别为53.06%和33.90%。【结论】陕西关中地区的肉品中存在产气荚膜梭菌污染,建议针对可能引起产气荚膜梭菌污染和大量繁殖的环节采取更有效的预防措施。【Objective】To understand the contamination and distribution of Clostridium perfringens（C.perfringens）in raw or cooked retailed meat in Guanzhong,Shaanxi,C.perfringens strains were isolated from collected retailed meat samples,and serotyped using established multi-PCR.【Method】A total of314 samples including fresh raw meat（chicken and pork）,cooked chicken claws,sausage and preserved pork were purchased randomly from supermarkets,farmer＇s markets and food stands in Xi＇an,Yangling and Wugong.Samples were isolated and purified as suspect C.perfringens strains.The isolates of C.perfringens were then confirmed using Gram staining,biochemical test,PCR amplification and serotyped by the developed multiplex PCR and genotyped by cpe gene detection.【Result】The isolation rate of C.perfringens was 45.86% and all isolates were classified as cpe-type A.C.perfringens identification rates in cooked meat samples and raw fresh meat samples were 50.91%and 43.14%,while the rates in chicken and pork were 53.06% and 33.90%,respectively.【Conclusion】This study demonstrated the presence of C.perfringens in meat products in Guanzhong,Shaanxi,and it is suggested to take more effective preventive measures to control contamination and mass proliferation of C.perfringens.

关 键 词：产气荚膜梭菌 多重PCR 零售肉品检测 菌株血清型 

分 类 号：S855.1[农业科学—临床兽医学]