不同培养条件下滨梅幼苗对NaCl胁迫的响应  

Responses of Prunus maritima Marshall seedlings to NaCl stress under different culturing conditions

作　　者：朱泓[1] 胡淑英[1] 张春红[1] 王小敏[1] 吴文龙[1] 李维林[1] 

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《西北农林科技大学学报（自然科学版）》2016年第11期70-76,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：海洋公益性行业科研专项(201505023);江苏省农业三项工程项目(SXGC(2015)332);江苏省木本油料高效栽培示范项目(LYSX[2015]14)

摘　　要：【目的】比较不同培养条件下滨梅幼苗耐盐特性差异,并筛选出耐盐能力强的滨梅品种,从而为滨梅耐盐性评价提供依据。【方法】以不同种源滨梅组培试管苗为试验材料,分别在组培和水培条件下,以0,100,200mmol/L NaCl进行盐胁迫处理,通过观察测定不同种源滨梅叶片盐害及生理生化指标,分析不同培养条件下滨梅幼苗对NaCl胁迫的响应情况。【结果】盐胁迫7d后,组培和水培滨梅幼苗叶片中Na+、丙二醛(MDA)、可溶性蛋白(SP)含量、相对电导率(REC)及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性均随NaCl处理浓度的升高显著增加;5种不同来源滨梅幼苗材料盐胁迫生理指标差异显著,其耐盐性强度表现为MI<MA1<NY<NJ<MA2,其中MA2可耐受200mmol/L NaCl,MI可耐受100mmol/L NaCl;组培条件下40d和水培条件下15d后,200mmol/L NaCl胁迫下NY、NJ、MA2所受盐害强度达到3级,MI、MA1所受盐害强度达到4级。【结论】不同浓度盐胁迫7d后组培和水培滨梅幼苗耐盐生理指标变化规律一致,滨梅组培试管苗可直接用于耐盐生理指标的测定和耐盐性评价。[Objective] This research investigated the influence of salt stress on physiological character- istics of Prunus maritima Marshall seedlings under different culturing conditions and selected new variety with strong salt tolerance. [Method] Five provenances of beach plum were treated with 0,100, and 200 mmol/L NaC1 under tissue and water culturing conditions to evaluate their salt-tolerance. [Result] With the increase of NaC1 concentration, MDA content, Na＋ content, total soluble protein content （SP）, relative conductivity,and activities of superoxide dismutase （SOD）,catalase （CAT） and peroxidase （POD） in leaves of seedlings were significantly increased under both tissue and water culturing conditions 7 days after treatment. The physiological indexes of the five provenances were significantly different with the salt-tolerance in order of MI〈MAI〈NY〈NJ〈MA2,in which MA2 had salt-tolerance of 200 mmol/L NaC1 and MI of 100 mmol/L NaC1. After 40 days treatment under tissue culturing and 15 days treatment under water culturing,200 mmol/L NaCI caused class HI salt injury to NY,NJ,and MA2,and class IV to MI and MA1. [Conclusion] Salt injury and physicochemical indexes changed similarly under different culturing conditions,indicating that the tube seedlings of beach plum can be used to investigate the physicochemical inde- xes for salt-tolerance evaluation.

关 键 词：滨梅 组织培养 水培 NACL胁迫 耐盐性 

分 类 号：Q494[生物学—生理学]