HPLC法同时测定复方葛根片中2种丹参酮类化合物的含量  

Simultaneous Determination of 2 Tanshinones in Compound Radix Pueraria Tablets by HPLC

作　　者：王飞飞[1] 杨文超[1] 于燕莉[2] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]济南军区总医院药剂科,山东济南250031

出　　处：《解放军药学学报》2016年第5期420-422,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立HPLC法测定复方葛根片中隐丹参酮和丹参酮ⅡA含量方法,以便更好的控制复方葛根片质量。方法采用HPLC法,色谱柱:SHMADZU VP-ODS柱(250 mm×4.6 mm,5μm);流动相:乙腈(A)-0.05%-磷酸水溶液(B)为流动相进行梯度洗脱:0~15 min,10%~50%A;15~25 min,50%~80%A;25~30 min,80%~90%A;检测波长:270 nm;柱温:30℃;流速:1.0 ml·min^(-1)。结果隐丹参酮在0.10~0.25μg范围线性关系良好(r=1),丹参酮ⅡA在0.11~0.275μg范围线性关系良好(r=0.999);平均回收率分别是99.83%、98.98%,RSD值分别是1.08%、0.81%。结论本方法简便可行,重复性好,可用于该制剂的质量检测。Objective To establish an HPLC method for the determination of the content cryptotanshinone and tanshinone ⅡAin compound radix puerariae tablets,in order to better control the quality of compound radix puerariae tablets. Methods HPLC was used. The chromatographic column was SHMADZU VPODS column（ 250 mm × 4. 6 mm,5 μm） with acetonitrile（ A） phosphoric acid-0. 05%-aqueous（ B） as the mobile phase using gradient elution. The detection wavelength was 270 nm,column temperature 30 ℃,and the flow rate was 1. 0 ml·min^-1.Results Cryptotanshinone showed a good linear relationship over the range of 0. 10-0. 25 μg（ r = 1）,and tanshinone ⅡAover the range of 0. 11-0. 275 μg（ r = 0. 999）. The average recovery was of 99. 83% and 98. 98%,respectively. RSD was 1. 08%,0. 81%. Conclusion This method is simple,reliable,reproducible and can be used for quality control of the preparation.

关 键 词：HPLC 复方葛根片 隐丹参酮 丹参酮ⅡA 

分 类 号：R927.2[医药卫生—药学]