Pathology and molecular characterization of recent Leucocytozoon caulleryi cases in layer flocks  

作　　者：Hae Rim Lee Bon-Sang Koo Eun-Ok Jeon Moo-Sung Han Kyung-Cheol Min Seung Baek Lee Yeonji Bae In-Pil Mo 

机构地区：[1]Avian Disease Laboratory,College of Veterinary Medicine,Chungbuk National University [2]Division of Basic Research,Bureau of Ecological Research,National Institute of Ecology

出　　处：《The Journal of Biomedical Research》2016年第6期517-524,共8页生物医学研究杂志（英文版）

基　　金：provided by the project of National Institute of Environment Research(No.2015080777)

摘　　要：Leucocytozoonosis was found in three layer farms in chickens with suspected fatty liver or fatty liver hemorrhagic syndrome in Korea between 2009 and 2011.These layer chicken flocks showed both mortality and decreased egg production for one or two weeks when they were between 59 and 82 weeks old.At the necropsy,the most prominent gross lesions were found in the liver,which was enlarged,had a fragile texture,exhibited yellowish discolorations,and had various hemorrhagic lesions.Tissue reactions associated with megaloschizonts specific for Leucocytozoon caulleryi were prominent upon microscopic examination of the liver without significant lipidosis.In addition,the ovaries and uterus were the most affected organs for Leucocytozoon caulleryi multiplication,which led to decreased egg productions.Molecular studies with formalin-fixed,paraffin-embedded tissues were performed in search of a partial region of the cytochrome b gene for hemosporidian parasites.Based on these results,the causal agent was determined to be closely related to Leucocytozoon caulleryi reported in Japan and Malaysia.In this study,we describe recently re-occurring leucocytozoonosis in layer chickens,which required histopathology for disease diagnosis.To prevent outbreaks and maintain chicken health and egg production,layer chickens need to be monitored for symptoms of leucocytozoonosis.Leucocytozoonosis was found in three layer farms in chickens with suspected fatty liver or fatty liver hemorrhagic syndrome in Korea between 2009 and 2011.These layer chicken flocks showed both mortality and decreased egg production for one or two weeks when they were between 59 and 82 weeks old.At the necropsy,the most prominent gross lesions were found in the liver,which was enlarged,had a fragile texture,exhibited yellowish discolorations,and had various hemorrhagic lesions.Tissue reactions associated with megaloschizonts specific for Leucocytozoon caulleryi were prominent upon microscopic examination of the liver without significant lipidosis.In addition,the ovaries and uterus were the most affected organs for Leucocytozoon caulleryi multiplication,which led to decreased egg productions.Molecular studies with formalin-fixed,paraffin-embedded tissues were performed in search of a partial region of the cytochrome b gene for hemosporidian parasites.Based on these results,the causal agent was determined to be closely related to Leucocytozoon caulleryi reported in Japan and Malaysia.In this study,we describe recently re-occurring leucocytozoonosis in layer chickens,which required histopathology for disease diagnosis.To prevent outbreaks and maintain chicken health and egg production,layer chickens need to be monitored for symptoms of leucocytozoonosis.

关 键 词：chicken leucocytozoonosis Leucocytozoon caulleryi layer chicken flocks histopathology megaloschizont 

分 类 号：S858.31[农业科学—临床兽医学]