黄芪注射液对降低围产期窒息仔鼠海马神经元氧化应激损伤的研究  

Research on radix astragali injection reducing oxidative stress on hippocampal neurons of asphyxia offspring rats during perinatal

作　　者：苏春宏[1] 孙雯[1] 毛丽丽[1] 周燕媚[1] 余琳[1] 陈艳红[1] Su Chunhong Sun Wen Mao Lili Zhou Yanmei Yu Lin Chen Yanhong(Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China)

机构地区：[1]广州医科大学附属第三医院妇产科,510150

出　　处：《中华产科急救电子杂志》2016年第2期114-117,共4页Chinese Journal of Obstetric Emergency（Electronic Edition）

基　　金：基金项目:广东省中医药局(20131269)

摘　　要：目的探讨黄芪注射液对降低围产期窒息仔鼠神经元氧化应激损伤的影响。方法将10周龄妊娠SD大鼠30只分为3组,每组10只,对照组:孕鼠饲养至19 d行剖宫取出仔鼠;窒息组:孕鼠饲养至19 d行子宫血管完全夹毕20 min后,建立窒息模型后,剖宫取出仔鼠;窒息+黄芪组:孕鼠于孕19 d行子宫血管完全夹毕20 min后,宫腔内注入黄芪注射液(10 ml/kg),剖宫取出仔鼠,迅速建立自主呼吸。取新生仔鼠海马组织,体外培养海马神经元细胞,检测神经元细胞存活率、超氧化物歧化酶(SOD)活力、丙二醛(MDA)和还原型谷胱甘肽(GSH)含量。结果窒息组、窒息+黄芪组的神经元细胞存活率分别为(56.78±14.62)%和(65.96±17.46)%,低于对照组(99.15±15.63)%,窒息组细胞存活率低于窒息+黄芪组,差异具有统计学意义(P<0.05)。窒息组、窒息+黄芪组和对照组SOD活力分别为(15.13±1.84)U/mg,(17.16±1.49)U/mg和(21.75±1.28)U/mg,GSH含量分别为(59.68±22.16)mg/g,(198.72±27.34)mg/g和(321.56±31.74)mg/g,窒息组、窒息+黄芪组SOD活力和GSH含量比对照组降低,窒息组SOD活力和GSH含量低于窒息+黄芪组,差异具有统计学意义(P<0.05)。窒息组、窒息+黄芪组和对照组MDA含量分别为(4.98±2.12)nmol/mg,(3.68±1.47)nmol/mg和(1.25±0.63)nmol/mg,窒息组、窒息+黄芪组MDA含量比对照组升高,窒息组MDA含量高于窒息+黄芪组,差异具有统计学意义(P<0.05)。结论围产期窒息导致仔鼠海马神经元细胞存活率降低和氧化应激损伤,黄芪注射液可降低活性氧对神经细胞的损伤,提高神经元细胞存活率。Objective To explore the radix astragali injection reducing oxidative stress on hippocampal neurons of asphyxia offspring rats during perinatal. Methods Thirty 10-week-old pregnant SD rats were divided into 3 groups （ 10 rats per group）. All the rats bad cesarean delivery on gestational clay of nineteenth. The rats had cesarean delivery without any interventions were in the control group. The rats had clipping uterine blood vessel for 20 minutes before cesarean delivery were in the asphyxia group. The rats had clipping uterine blood vessel for 20 minutes and then injected radix astragali （ 10 ml/kg） into the uterus before cesarean delivery were in the asphyxia ± radix group. The hippocampal neurons of offspring rats were cultivated in vitro after birth. Meanwhile, the cell survival rate, the activity of superoxide dismutase （ SOD）, and the amount of malondialdehyde （MDA） and glutathione （ GSH ） were detected. Results The cell survival rate of asphyxia group and asphyxia ± radix group were （56. 78 ± 14. 62）% and （65.96 ± 17. 46）%, which were significant lower than that of control group （99.15 ± 15.63 ） % ; And the cell survival rate of asphyxia group was also significant lower than that of asphyxia ± radix group ; all P values were less than 0. 05. The SOD activity of asphyxia group, asphyxia ± radix group and control group were （15.13 ±1.84） U/mg, （17.16 ±1.49） U/rag and （21.75 ±1.28） U/mg, respectively; The GSH content of the three groups were （ 59. 68 ± 22. 16） mg/g, （ 198. 72 ± 27. 34） mg/g and （321.56 ± 31.74） mg/g, respectively. The SOD activity and GSH content of asphyxia group, asphyxia ＋ radix group were significant lower than those of control group; and SOD activity, GSH content of asphyxia group were lower than those of asphyxia ＋ radix group, there were significant difference, all P values were less than 0. 05. The MDA content of asphyxia group, asphyxia ＋ radix group were （4. 98 ± 2. 12） nmol/mg, （3.68 ± 1.47） nmol/mg

关 键 词：黄芪 海马 神经元 妊娠 动物 窒息 氧化性应激 

分 类 号：R714.7[医药卫生—妇产科学]