miR-916a调控SOCS6促进HBx-HepG2细胞生长  

miR-196a promotes cell growth of HBx-HepG2 via targeting SOCS6

作　　者：余桂芳[1] 陈树娣[1] 陈雪竹[1] 侯开连[1] 梁敏[1] 

机构地区：[1]广州医科大学附属第五医院肿瘤科,广东广州510700

出　　处：《山东大学学报（医学版）》2016年第12期14-19,共6页Journal of Shandong University:Health Sciences

基　　金：广东省科技计划(20130319c)

摘　　要：目的探讨HBx-HepG2细胞中miR-196a对细胞因子信号抑制物6(SOCS6)表达的调控作用以及对细胞生长的促进作用,并研究其潜在的分子作用机制。方法利用qRT-PCR检测miR-196a以及SOCS6 mRNA的表达水平;Western blotting检测SOCS6蛋白表达水平;CCK-8和集落形成实验检测细胞的生长;采用双荧光素酶报告实验验证miR-196a的靶基因。结果与正常人肝细胞(L02)相比,miR-196a在HBx-HepG2细胞中过量表达(P<0.05)。miR-196a过表达可促进HBx-HepG2细胞生长;miR-196a表达下调可抑制HBx-HepG2细胞生长,与对照组相比差异具有统计学意义(P<0.05)。与正常人肝细胞(L02)相比,SOCS6 mRNA在HBx-HepG2细胞中表达下调(P<0.05);miR-196a可以通过作用于SOCS6的3'UTR区负向调控其表达,并且SOCS6的过表达可以抑制HBx-HepG2细胞的生长。结论 miR-196a可以通过靶向负调控SOCS6基因进而促进HBx-HepG2细胞的生长。Objective To explore the effect of miR-196 a on cell growth in HBx-HepG2 cells,and to explore its underlying molecular mechanism. Methods The expressions of miR-196 a and SOCS6 mRNA were detected with QRTPCR,and the protein expression of SOCS6 was determined with Western blotting. The cell growth in HBx-HepG2 cells was evaluated with CCK-8 and colony formation assay. The target gene of miR-196 a was validated with dual luciferase reporter experiment. Results The expression of miR-196 a was significantly higher in HBx-HepG2 cells than in normal human liver cells（ L02）（ P〈0. 05）. Over-expression of miR-196 a enhanced cell growth in HBx-HepG2 cells（ P〈0. 05）,while knockdown of miR-196 a suppressed cell growth（ P〈0. 05）. SOCS6 was downregulated in HBx-HepG2 cells compared with L02 cells（ P〈0. 05）,and miR-196 a downregulated the expression of SOCS6 via targeting 3＇UTR of SOCS6. Overexpression SOCS6 in HBx-HepG2 cells promoted cell growth. Conclusion miR-196 a enhances cell growth via down-regulation of SOCS6 in HBx-HepG2 cells.

关 键 词：miR-196a HBx-HepG2细胞 细胞生长 细胞因子信号抑制物6 

分 类 号：R574[医药卫生—消化系统]