两种核酸检测方法在猪源与人源戊型肝炎病毒监测中的应用  

Feasibility evaluation of two PCR methods in pig and human derived HEV surveillance

作　　者：田华[1] 张雪峰[1] 翟祥军[1] 付建光[1] 鲍倡俊[1] 

机构地区：[1]江苏省疾病预防控制中心,南京210009

出　　处：《江苏预防医学》2016年第6期660-662,669,共4页Jiangsu Journal of Preventive Medicine

基　　金：江苏省科技支撑计划(社会发展)项目(BE2013723);江苏省预防医学项目(Y2012067)资助

摘　　要：目的评价两种核酸检测方法在猪源与人源的戊型肝炎病毒（HEV）监测应用的可行性。方法收集江苏地区急性戊肝病例血液标本和屠宰场生猪胆囊标本，采用Real—timeRT-PCR法和RT-PCR法进行RNA检测，对阳性检出情况和核酸序列比对结果进行分析。结果两种方法监测急性戊肝病例和猪胆囊标本的结果完全一致，患者血清HEVRNA检出阳性率为38．94％，猪胆汁标本HEVRNA检出阳性率为4．67％。HEV-4型为2014年本省主要人源和猪源毒株，且病毒核苷酸序列同源性为83％～97％。结论Real—timeRT—PCR法快捷、简便性和RT-PCR的分型、序列分析作用相结合，可在HEV感染和传染源监测中发挥重要作用。Objective To evaluate the feasibility of 2 PCR methods in pig and human-derived HEV surveillance. Methods Real-time RT-PCR and RT-PCR were used to analyze RNA extracted from the HEV patients＇ serum and pig gallbladders collected from different regions of Jiangsu province; the detection rates and sequences of RT-PCR products were analyzed. Results The positive rates of Real-time RT-PCR and RT-PCR were both 38.94% for HEV patients＇s serums and 4.67% for pig gall-bladders,with 100% consistency. Sequence analysis showed that HEV-4 was dominant type in Jiangsu province for both human and pig derived viral isolates, demonstrating 83%-97% homology for nucleic acid sequences. Conclusion Real-time RT- PCR is fast and easy to operate ,which is suitable for HEV preliminary screening. RT-PCR has greater advantages in genotyping and sequence analysis. The combination of Real-time RT-PCR and RT-PCR would play an important role in HEV surveillance.

关 键 词：戊肝病毒 Real—timeRT—PCR RT-PCR 序列分析 人兽共患病 

分 类 号：R512.6[医药卫生—内科学]