新型多级孔生物玻璃对人成骨细胞增殖和分化的促进作用  

Promotive effect of a new type of mesoporous bioactive glass on proliferation and differentiation of human osteoblasts

作　　者：齐佳[1] 孙新华[1] 黄粲[2] 娄译心 汪洋[1] 申玉芹[3] 

机构地区：[1]吉林大学口腔医院正畸科吉林省牙发育及颌骨重塑与再生重点实验室,吉林长春130021 [2]吉林大学食品科学与工程学院食品质量与安全系,吉林长春130062 [3]吉林大学口腔医院牙周病科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2016年第6期1049-1053,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金面上项目资助课题(81371153);吉林省科技厅自然科学基金项目资助课题(20150101173JC);吉林大学白求恩前沿交叉学科创新项目资助课题(20131080031)

摘　　要：目的:探讨一种新型的以地中海海绵为模板合成的多级孔生物玻璃(MBG)对人源性成骨样细胞系MG63增殖和分化的影响,阐明该生物活性玻璃的成骨分化作用。方法:扫描电镜观察MBG材料孔架结构;取对数生长期MG63细胞分别给予MBG浸提液(MBG组)、普通生物玻璃(NBG组)和空白培养基(空白对照组),共培养1、3和5d。MTT法检测各组MG63细胞的增殖率,酶联免疫法检测MG63细胞中碱性磷酸酶(ALP)的活性,实时定量PCR法检测培养24h时各组MG63细胞中成骨相关基因Sp7mRNA及Runx2mRNA的相对表达水平。结果:扫描电镜下观察,MBG为多孔支培养架结构;培养1、3和5d时MBG组MG63细胞增殖率高于空白对照组和NBG组(P<0.01);1、3和5d时,MBG组MG63细胞中ALP活性高于空白对照组和NBG组(P<0.01);培养24h后MBG组MG63细胞中Sp7 mRNA相对表达水平高于(P<0.01),但Runx2mRNA相对表达水平与空白对照组和NBG组比较差异无统计学意义(P>0.05)。结论:与NBG比较,该新型MBG具有细胞毒性小和可促进成骨细胞分化的优势。Objective：To investigate the effect of a new type of mesoporous bioactive glass with a hierarchical pore structure on the proliferation and differentiation of osteoblasts,and to clarify its osteogenetic differentiation effect.Methods：The MBG structure was observed by scanning electron microscope（SEM）;the MG63 cells at logarithmic growth phase were selected and divided into MBG group（cultured with MBG）,NBG group（cultured with NBG）and blank control group（cultured with blank cultrue medium）,and they were cultured for 1,3,and5 d.The proliferation rate of MG63 cells was analyzed by MTT method;enzyme-linked immunoassay method was used to analyze the alkaline phosphates（ALP）activity in MG63cells;real-time PCR was used to detect the relative expression levels of Sp7 mRNA and Runx2 mRNA in MG63 cells after cultured for 24 h.Results：The SEM results showed that the MBG had porous scaffold structure.Compared with blank control group and NBG group,the proliferation rates of MG63 cells in MBG group at 1,3,and 5 d were significantly increased（P〈0.01）.Compared with blank control group and NBG group,the activities of ALP in the MG63 cells in MBG group at 1,3,5d were significantly increased（P〈0.01）.Compared with blank control group and NBG group,the relative expression level of Sp7 mRNA in the MG63 cells in MBG group was significantly increased 24 hafter culture（P〈0.01）,while the relative expression level of Runx2 mRNA in the MG63 cells in MBG group had no significant differences（P〉0.05）.Conclusion：Compared with NBG,the MBG presents the promotive effect on the proliferation and differentiation of osteoblasts in vitro with low cytotoxicity.

关 键 词：生物活性玻璃 成骨细胞 碱性磷酸酶 细胞增殖 分化 

分 类 号：R318.5[医药卫生—生物医学工程]