18～60岁健康人群肺炎链球菌IgG抗体水平调查  

Investigation of pneumococcal IgG antibody levels on healthy population from 18 to 60 years age

作　　者：张轶[1] 熊明郁[1] 张新庄[1] 任珍芸[1] 刘倩[1] 张丽芝[1] 任克明[1] 王玺[1] 陈晓航[1] 王剑虹[1] 沈荣[1] 

机构地区：[1]兰州生物制品研究所有限责任公司第三研究室甘肃省疫苗工程技术研究中心,甘肃兰州730046

出　　处：《微生物学免疫学进展》2016年第6期70-75,共6页Progress In Microbiology and Immunology

摘　　要：目的了解18~60岁健康人群肺炎链球菌IgG抗体水平,为肺炎链球菌性疾病的预防与控制提供参考依据。方法选取甘肃、宁夏健康献浆员为调查对象,分为18~30、31~40、41~50、51~60岁4个年龄组,共调查347人。采用WHO推荐的标准化酶联免疫吸附试验检测12种肺炎链球菌血清型IgG抗体水平,并计算IgG抗体阳性率和几何平均浓度（Geometric mean concentration,GMC）。结果质控血清QC907的IgG抗体浓度均值与参考值之间的误差百分数均未超过±40%,测定值符合其参考值范围,各血清型抗体浓度检测结果的CV均〈30%。347份血清样品肺炎链球菌IgG抗体总阳性率为64.8%~96.0%,GMC为0.37~2.24μg/m L。不同血清型间IgG抗体GMC差异具有统计学意义（P〈0.05）。不同年龄组同种血清型IgG抗体阳性率及GMC均无统计学意义（P〉0.05）。除19F型外,不同地区同种血清型IgG抗体阳性率均有统计学意义（P〈0.05）;除4、7F、14和18C型外,不同地区同种血清型IgG抗体GMC间差异均有统计学意义（P〈0.05）。结论 18~60岁健康人群肺炎链球菌IgG抗体水平普遍较高,对肺炎链球菌主要流行血清型均具有一定的保护力。Objective To investigate Streptococcus pneumoniae IgG antibody levels on healthy population from 18 to 60 years old, in order to provide a reference in prevention and control of pneumocoecal diseases. Methods A total of 347 healthy plasma donors were divided into 18-30, 31-40, 41-50 and 51-60 age groups from Gansu and Ningxia area. The IgG levels to 12 serotypes were measured by PnPS ELISA in 347 plasma samples, and furthermore, the sero-prevalence and geometric mean concentration { GMC ） of samples determined too. Results The average values for IgG concentrations measured by Qc907 （ Quality control serum） were within a deviation of ±40% with the reference values, which were within the range of reference, and all CV 〈30%. The positive rate and the range of GMC for Streptococcus pneumoniae IgG were 64.8% -96.0% and 0.37-2.24 μg/mL, separately, from 347 samples. The GMCs for IgG had a statical difference （ P〈 0.05 ） among the different serotypes involved in the study. The sero-prevalences and GMCs for IgG had no statistical differ- ence （ P〉0.05 ） in the same serotypes among the different age groups. The positive rate for IgG had a statical difference （P〈0.05） in the same serotypes from the different areas, except for serotype 19. The difference of GMCs for IgG had a statistical difference （ P〈0.05 ） in the same serotypes from the different areas except for serotype 4, 7F, 14 and 18C. Con- clusion The Streptococcus pneumoniae IgG antibody levels were generally high on healthy population from 18 to 60 years age, on which a certain of protection could be provided in control of the epidemically predominant strain in Streptococcuspneumoniae.

关 键 词：肺炎链球菌 抗体水平 调查 酶联免疫吸附试验 

分 类 号：R446.6[医药卫生—诊断学]