7种甘肃道地药材凝血活性初步分析  

The Preliminary Analysis of Agglutinating Activity of Seven Kinds of Genuine Medicinal Materials in Gansu

作　　者：袁毅君[1] 赵丽[1] 焦成瑾[1] 陈荃[1] 

机构地区：[1]天水师范学院生物工程与技术学院,甘肃天水741001

出　　处：《天水师范学院学报》2016年第5期15-18,共4页Journal of Tianshui Normal University

基　　金：国家自然基金项目"基于抑制硫氧还蛋白还原酶活性的甘肃道地药材抗癌成分筛选"(81360619)阶段性成果

摘　　要：目的:检测7种甘肃道地药材对鸡、兔红细胞的凝集活性。方法:将7种药材分别用PBS(磷酸缓冲液)浸提,浸提液分别对兔和鸡红细胞进行凝集活性测定,以检测7种药材的最低效价。结果:7种中药浸提液对红细胞表现不同的凝集活性,具有较高凝集活性的为防风对兔红细胞效价23;大黄对鸡红细胞效价25.结论:7种中药均有不同的凝集活性,同种中药对不同红细胞的凝集活性不同。Objective： To test the agglutinating activity about seven kinds of Genuine Medicinal Materials in Gansu react with the red blood cells of chicken and rabbit respectively. Methods： Selected seven medicinal herbs were ex？tracted with PBS, then extracts agglutinating activity were detected with the red blood cells from rabbit and chicken respectively, determined the lowest titer of the herbs. Results： The agglutination activities of the lectins varied in the seven herbs extracts, and the higher agglutinating activity of the herb lectins in the two different blood cells are fol-lows： the titer of extracts from Radix Saposhnikoviae with the rabbit red blood cells is 23; but the titer of extracts from Radix et Rhizoma Rhel with chicken red blood cells is 25. Conclusion： Seven kinds of Chinese herbs present better agglutinating activity, the same Chinese herbs has different agglutinating activity with different erythrocyte samples.

关 键 词：道地药材 凝血活性 凝集素 效价 

分 类 号：R932[医药卫生—生药学]