枳术颗粒对慢性萎缩性胃炎大鼠胃组织COX-2和VEGF表达的影响  

Effect of Zhizhu Granules on Expressions of VEGF and COX-2 in Rat Model with Chronic Atrophic Gastritis

作　　者：赵凡[1] 唐德才[2] 张硕[2] 郭秀霞[2] 尹刚[2] 戴建国[2] 

机构地区：[1]南京中医药大学翰林学院,江苏泰州225300 [2]南京中医药大学,南京210046

出　　处：《中国实验方剂学杂志》2016年第24期150-155,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省中医药管理局课题项目(HZ07066);南京中医药大学基础研究与重点培育项目(10XJC01);南京中医药大学青年基金项目(13XZR07)

摘　　要：目的:观察枳术颗粒对慢性萎缩性胃炎(CAG)大鼠的疗效,并探讨其作用机制。方法:选取已建立CAG模型的大鼠,分为模型组,胃复春(0.86 g·kg-1)组,枳术颗粒高、中、低剂量(20,10,5 g·kg-1)组。用蛋白免疫印迹法(Western blot),免疫组化及实时荧光定量PCR(Real-time PCR)方法检测胃组织中环氧合酶-2(COX-2),血管内皮生长因子(VEGF)蛋白及mRNA的表达。结果:与正常组比较,模型组大鼠胃黏膜腺腔结构不完整,炎细胞浸润黏膜全层,体重增长率降低,COX-2,VEGF蛋白及mRNA表达显著增加(P<0.01);与模型组比较,枳术颗粒高、中、低剂量组及胃复春组均显著增加CAG大鼠体重增长率(P<0.01),下调大鼠胃组织COX-2,VEGF蛋白及mRNA表达(P<0.01);与胃复春组比较,枳术颗粒高、中、低剂量组均显著增加CAG大鼠体重增长率(P<0.01),高剂量组可下调大鼠胃组织COX-2,VEGF蛋白及mRNA表达,但无明显差异。结论:枳术颗粒可加快大鼠体重增长,使CAG大鼠胃黏膜组织得到改善和恢复。其作用机制可能为下调胃黏膜破坏因子COX-2,VEGF的表达,起到预防及治疗作用。Objective： To observe the effect of Zhizhu granules on the rat model with chronic atrophic gastritis（ CAG）. Method： The rat model with chronic atrophic gastritis was established,and divided into model group,positive control group（ Weifuchun,0. 86 g·kg- 1）,and Zhizhu granules high,medium and low-dose（ 20,10,5 g·kg- 1） groups. The mRNA and protein expressions of cyclooxygenase-2（ COX-2） and vascular endothelial growth factor（ VEGF） were detected by Western blot,immunohistochemical（ IHC） and Real-time PCR method.Result： Compared with normal group,model group showed incomplete gastric mucosa gland cavity structure,obvious inflammatory cell infiltration,significant decreasing in weight growth rate,and significant increase in COX-2 and VEGF protein and mRNA expressions（ P〈0. 01）. Compared with model group,Zhizhu granules high,medium and low-dose groups and Weifuchun group showed significant increase in CAG rats＇ body weight growth rate（ P〈0. 01）,decrease in COX-2,VEGF protein and mRNA expressions in rat gastric tissues（ P〈0. 01）. Compared with Weifuchun group,Zhizhu granules high,medium and low-dose groups showed significant increase in CAG rats＇ body weight growth rate（ P〈0. 01）,high-dose group could decrease the expression of COX-2,VEGF protein and mRNA expressions in rat gastric tissues,with no significant difference. Conclusion： Zhizhu granules can increase the growth of the body weight of rats,and also alleviate pathological morphology in stomach tissues.Zhizhu granules show the preventive and therapeutic effects by down-regulating the expressions of COX-2 and VEGF.

关 键 词：枳术颗粒 慢性萎缩性胃炎 环氧合酶2 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]