乙醇法提取苦瓜皂苷工艺择优研究  

Research on the extraction process of the saponin from balsam pear

作　　者：张宏梅[1] 李晓光[1] 

机构地区：[1]吉林医药学院药学院,吉林吉林132013

出　　处：《吉林医药学院学报》2016年第6期433-435,共3页Journal of Jilin Medical University

摘　　要：目的采用正交实验法优化苦瓜中皂苷的提取工艺。方法以乙醇为提取溶剂,采用乙醇回流提取法,对苦瓜中的皂苷进行提取,通过正交实验,对提取温度、乙醇浓度、提取时间、料液比、提取次数5个因素进行了考察探索出提取苦瓜皂苷的最佳工艺条件。结果苦瓜皂苷的最佳提取条件为:乙醇浓度75%,提取温度70℃,料液比1∶30,提取时间2.5 h,提取次数2次,皂苷平均得率为0.65%。结论提取工艺稳定、可行、有较高的提取率,并为苦瓜皂苷的进一步的深入研究奠定了一定基础。Objective To optimize the extraction process of the saponin from balsam pear by orthogonal design . Methods In orthogonal experiment ,ethanol concentration ,extraction temperature ,extraction time and solid-liquid ra-tio and extraction times five factors were investigated .Results The optimum extraction conditions of balsam pear saponin was：75%ethanol,temperature 70 ℃,material ratio 1∶30,times of extraction 2.5 h,times of extraction 2. Average content of saponin was 0.65%.Conclusion The method is simple,convenient,easily and suitable for the determination of the saponin from balsam pear .

关 键 词：苦瓜 皂苷 回流提取 

分 类 号：R286.0[医药卫生—中药学]