基于谱-效相关的雷公藤抗炎药效物质的研究  

Study on Effective Components of Roots of Tripterygium wilfordii Based on Spectrum-effect Relationships Analysis

作　　者：董捷鸣 续畅[1] 宫嫚[2] 赵庆国[2] 王伽伯[2] 肖小河[2] 马致洁[1] 浦仕彪[1,3] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050 [2]解放军302医院中西医结合研究中心,北京100039 [3]云南中医学院中药学院,云南昆明650200

出　　处：《中国现代中药》2016年第12期1583-1588,共6页Modern Chinese Medicine

基　　金：云南省应用基础研究面上项目(2014FZ072);北京友谊医院院启动项目(YYDQKT2014-20);北京中医管理局项目(QN2014-18);首都中医药研究专项(15ZY04)

摘　　要：目的:基于谱-效相关分析方法,对雷公藤体外抗炎药效物质进行初步筛选。方法:运用LC-Q/TOFMS技术表征不同产地雷公藤的化学成分并初步指认其成分;以小鼠骨髓来源巨噬细胞(BMDM)为模型,检测细胞上清液中的白介素-6和白介素-10及诱导性一氧化氮合成酶,评价雷公藤的体外抗炎效应。采用相关回归分析方法分析效应与成分之间的关系。结果:指认出雷公藤的10个有效成分;确认BMDM模型可以有效评价雷公藤体外抗炎效应,并通过简单及多元相关回归分析可知雷公藤甲素、雷公藤晋碱应对雷公藤抗炎效应有较大相关。结论:雷公藤晋碱可能与雷公藤抗炎效应相关性较高。Objective： Based on the spectrum-effect relationships analysis,to study the in vitro anti-inflammatory effect of the roots of Tripterygium wilfordii Hook. f. Methods： LC-Q / TOF-MS technology was used to characterize the chemical composition of the roots of T. wilfordii samples come from different regions. And macrophages（ BMDM） as carrier,interleukin 6（ IL-6）,IL-10 and nitric oxide synthase（ NOS） as indicators,evaluating the anti-inflammatory effect of the roots of T. wilfordii in vitro. Results： Ten kinds of main components were identified. Bone marrow-derived macrophage（ BMDM） as carrier was able to evaluate the anti-inflammatory effect in vitro. Through the simple and multiple regression analyses,triptolide and wilforgine could be the anti-inflammatory substances of the roots of T. wilfordii. Conclusion： Wilforgine may be a higher effect component of the roots of T. wilfordii.

关 键 词：雷公藤 抗炎效应 谱-效相关 巨噬细胞 雷公藤晋碱 

分 类 号：R285.5[医药卫生—中药学]