牛副流感病毒3型在不同细胞中的复制动力学分析  

Replication kinetics of bovine parainfluenza virus type 3 in various cells

作　　者：赵红丽[1] 刘智慧[1] 李婷婷[1] 黄晓娜[1] 初佳芮 陶冬[1] 张楠[1] 柏丛[1] 霍琦[1] 刘辉[1] 

机构地区：[1]长春生物制品研究所有限责任公司,吉林长春130062

出　　处：《中国生物制品学杂志》2016年第12期1255-1257,1261,共4页Chinese Journal of Biologicals

摘　　要：目的对牛副流感病毒3型(bovine parainfluenza virus type 3,BPIV3)在Vero-E6、MRC-5和MDBK 3种细胞中的复制动力学进行分析。方法将BPIV3分别接种至Vero-E6、MRC-5和MDBK 3种细胞,盲传3代,逐日观察细胞病变(CPE)情况;采用直接免疫荧光抗体法检测3种细胞盲传3代后BPIV3的增殖情况;将病毒滴度为1.0×104TCID50/ml的BPIV3分别接种至MDBK和Vero-E6单层细胞中,培养7 d,逐日测定各细胞中的病毒滴度。结果BPIV3在MDBK细胞中盲传1代即可观察到明显的CPE;在Vero-E6细胞中盲传2、3代可见CPE;在MRC-5细胞中盲传3代未见CPE。在MDBK细胞中盲传3代可检测到较强荧光信号;在Vero-E6细胞中盲传3代荧光强度较弱;在MRC-5细胞中盲传3代未检测到荧光信号。BPIV3在MDBK和Vero-E6细胞中分别于培养第5和6天时病毒滴度达峰值,分别为1.2×1010和1.0×105 TCID50/ml。结论 BPIV3在MDBK细胞中的复制动力强于Vero-E6细胞,最适用于培养该病毒,MRC-5细胞不适用于培养BPIV3。Objective To analyze the replication kinetics of bovine parainfluenza virus type 3（BPIV3）in Vero-E6, MRC-5 and MDBK cells. Methods BPIV3 was inoculated to Vero-E6, MRC-5 and MDBK cells respectively for three blind passages, and observed for CPE daily. The proliferation of BPIV3 was determined by direct immunofluorescence antibody assay. The BPIV3 at a titer of 1. 0 × 10^4TCID50/ ml was inoculated to MDBK and Vero-E6 cell monolayer respectively,cultured for 7 d and determined for titer daily. Results BPIV3 caused obvious CPE in MDBK cells after one blind passage, while in Vero-E6 cells after two and three blind passages. However, the virus caused no CPE in MRC-5 cells after three blind passages. Strong fluorescence signal was observed in MDBK cells in which BPIV3 was subcultured for three blind passages, while weak fluorescence signal in Vero-E6 cells, and no fluorescence signal in MRC-5 cells. The titers of BPIV3 reached the peak values of 1. 2 × 10^10 and 1. 0 × 10^5TCID50/ ml on days 5 and 6 after culture in MDBK and Vero-E6 cells, respectively. Conclusion The replication kinetics of BPIV3 was stronger in MDBK cells than in VeroE6 cells, indicating that MDBK cells were suitable for culture of BPIV3, while MRC-5 cells were unsuitable.

关 键 词：牛副流感病毒3型 VERO-E6细胞 MRC-5细胞 MDBK细胞 复制动力学 

分 类 号：S852.653[农业科学—基础兽医学]