大鼠衰老过程中勃起功能改变与去乙酰化酶SIRT1的关系研究  

Aging-related change of erectile function and the expression of SIRT1

作　　者：余文[1] 徐志鹏[1] 车晓艳[1] 陈贇[1] 戴玉田[1] 

机构地区：[1]南京大学医学院附属鼓楼医院男科,江苏南京210008

出　　处：《中华男科学杂志》2016年第12期1077-1082,共6页National Journal of Andrology

基　　金：国家自然科学基金(80772175)~~

摘　　要：目的:研究衰老过程中大鼠勃起功能变化、抗衰老蛋白SIRT1表达及其相关因子改变,探索SIRT1与老年性勃起功能障碍(ED)发生的关系。方法:SD大鼠按不同月龄分组,利用电刺激大鼠盆腔星状神经节法测定阴茎海绵体内压(ICP),颈动脉穿刺测定平均动脉压(MAP),ICP/MAP作为勃起功能的评价指标;马松染色观察胶原纤维变化;Western印迹测定不同月龄大鼠阴茎海绵体中SIRT1、P53及FOXO3a的表达变化;NO、c GMP检测试剂盒测定阴茎海绵体中NO、c GMP含量。结果:随月龄增长,大鼠勃起功能(ICP/MAP)在8月龄达到峰值,其后随月龄增长降低。c GMP含量变化与勃起功能一致。随月龄增加,大鼠阴茎海绵体中胶原纤维增多。SIRT1的表达随月龄增加不断降低;而凋亡因子P53、氧化应激因子FOXO3a的表达随月龄增加不断上升。结论:NO/c GMP通路活性降低、凋亡及氧化应激可能是衰老导致ED的原因。抗衰老蛋白SIRT1与其调控的凋亡、氧化应激因子改变与勃起功能一致,推测SIRT1与老年性ED的发病有关。Objective： To explore aging-related changes in erectile function and the expressions of SIRT1 and other relevant factors in rats. Methods： We divided 40 male SD rats into four age groups of equal number： 2-month-old （2 mo） , 8-month-old （8 mo）, 14-month-old （14 mo）, and 20-month-old （20 too）, measured the intracavernous pressure （ICP）, mean arterial pressure （MAP）, and ICP/MAP ratio by electrostimulation of the cavernous nerve, evaluated fibrosis in the corpus cavernosum by Masson＇s trichrome staining, detected the expressions of SIRT1, P53, and FOXO3a by Western blot, and determined the levels of NO and cGMP using the NO/eGMP kit. Results ： Both the ICP/MAP ratio and the cGMP level were elevated with aging, reaching the peak at 8 months and then gradually decreased. Masson staining showed an aging-related increase of collagen fibers in the corpus cavernosum. The expression of SIRT1 was reduced while those of P53 and FOXO3a increased with aging. Conclusion ： Aging-related erectile dys- function may be attributed to the reduced activity of the NO/cGMP pathway, apoptosis and oxidative stress, and SIRT1 may play a role in aging-related erectile dysfunction.

关 键 词：勃起功能障碍 沉默信息调节因子2相关酶1 衰老 凋亡 氧化应激 大鼠 

分 类 号：R698.1[医药卫生—泌尿科学]