枸杞多糖通过氧化应激途径促海绵体神经再生的实验研究  

The neuroprotective effect of lycium barbarum polysaccharide after cavernous nerve injury is mediated by antioxidative mechanism

作　　者：丁协刚[1] 刘波[1,2] 李世文[1] 王行环[1] 

机构地区：[1]武汉大学中南医院泌尿外科,湖北武汉430071 [2]湖北医药学院附属十堰市太和医院泌尿外科,湖北十堰442000

出　　处：《现代泌尿外科杂志》2016年第12期960-963,共4页Journal of Modern Urology

基　　金：国家自然科学基金(No.81100492)

摘　　要：目的探讨氧化应激在枸杞多糖(lycium barbarum polysaccharides,LBP)促海绵体神经(cavernous nerves,CN)钳夹损伤后再生中的作用。方法 84只雄性SD大鼠随机平均分成3组,即假手术组、CN损伤对照组、LBP处理组。LBP处理组于术后第1天开始,连续灌胃2周。分别于术后1周、2周、4周、12周每组随机取7只大鼠行血清丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性及谷胱甘肽过氧化物酶(GPX)活性测定,术后12周电刺激CN测定海绵体内压,随后取CN进行甲苯胺蓝染色记录神经有髓鞘轴突数目。结果在术后第1、2和第4周,LBP组超氧化物歧化酶和谷胱甘肽过氧化物酶活性显著高于假手术组及损伤对照组;在术后第2周和第4周,LBP处理组MDA含量明显低于假手术组及损伤对照组;术后12周LBP处理组平均海绵体内压、CN横切面有髓鞘轴突数目均明显高于损伤对照组,但均低于假手术组。结论 LBP可通过减轻CN钳夹损伤后的氧化应激来促进CN神经再生及勃起功能的恢复。ObjectiveTo investigate the neuroprotective effect of lycium barbarum polysaccharides （LBP） mediated by antioxidative mechanism after cavernous nerve （CN） injury. MethodsA total of 84 male SD rats were randomly divided into three groups：sham operation group （sham group），bilateral CN crushed group （control group），bilateral CN crushed with an oral administration of LBP 1 day after CN injury for 2 weeks （LBP group）. Malondialdehyde （MDA），super oxide dismutase （SOD） and glutathione peroxidase （GPX） were evaluated in week 1，2，4，and 12. Erectile function was assessed by cavernous nerve electrostimulation in week 12 and nerve regeneration was assessed by toluidine blue staining of CN. ResultsSOD and GPX activities in the LBP group were significantly higher than those in the sham and control group in week 1，2 and 4. MDA level in LBP group was significantly lower than that in the sham and control group in week 2 and 4. In week 12，the mean intracavernous pressure （ICP），and the number of myelinated axons of CNs in LBP group were significantly higher than those in the control group，but lower than those in the sham group. ConclusionLBP could promote nerve regeneration and erectile function recovery after CN crush injury by reducing antioxidative reaction.

关 键 词：枸杞多糖 勃起功能障碍 海绵体神经 

分 类 号：R698[医药卫生—泌尿科学]