重组人p53腺病毒对糖尿病小鼠糖代谢的影响  

Effect of rAd-p53 on glucose metabolism in diet-induced and genetic T2DM mice

作　　者：段薇[1] 项芬芬 高英慧[1] 冯一丹[1] 张学梅[2] 

机构地区：[1]大连大学附属中山医院内分泌科,116001 [2]大连大学医学院

出　　处：《中国糖尿病杂志》2016年第12期1100-1104,共5页Chinese Journal of Diabetes

基　　金："十二五"国家科技支撑计划项目(2012BAK25B00)

摘　　要：目的观察重组人p53腺病毒(rAd-p53)对两种T2DM模型鼠血糖的影响,探讨rAd-p53对糖代谢的影响及机制。方法制备HFD/STZ鼠,将HFD/STZ鼠和db/db鼠各随机分为阴性对照(rAd)组、阳性对照(胰岛素)组和rAd-p53给药组,每组8只。分别依次予rAd(4×10^(10)/ml)、中效胰岛素(1 U/kg)、rAd-p53(4×10^(10)/ml)。每72 h给药1次,连续给药3次,6周后检测各组FPG、腹腔糖耐量(IPGTT)和C-P水平。结果 rAd-p53改善HFD/STZ鼠的FPG、IPGTT,并增加C-P水平(P<0.05);但以同等剂量rAd-p53和胰岛素处置db/db鼠时,对其血糖和C-P改善不明显(P>0.05),使用3倍剂量胰岛素才能改善其血糖水平(P<0.05)。结论 rAd-p53可改善HFD/STZ鼠FPG、IPGTT和C-P水平,可能与其改善胰岛β细胞分泌缺陷、增加胰岛素释放有关。Objective To explore the possible mechanism of rAd-p53 involved in glucose metabolism by observing the different effects of rAd-p53 on high-fat diet （HFD） fed with low-dose streptozotocin （STZ） treated （HFD/STZ） mice （fl-cell dysfunction model） and db/db mice （genetic insulin resistance model）. Methods The established HFD/STZ and db/db diabetic mice were respectively and randomly divided into three groups （n=8 each） ： rAd （4x10^10/ml） treated group （negative control）, intermediate- acting insulin（1 U/kg） injected group （positive control） and rAd-p53 （4x10^10/ml） treated group. After one week from the last STZ injection, intraperitoneal administration of rAd-p53 was begun once every 72 hours for three injections. 6 weeks later, fasting plasma glucose （FPG）, IPGTT and C-peptide levels were detected. Results Compared with negative control group, rAd-p53 could significantly improve FPG, IPGTT and C-peptide in HFD/STZ mice（P〈0. 05）. Insulin and rAd-p53 had ＇no significant effect on the levels of FPG, IPGTT and C-peptide in db/db mice（P〈0.05）. Only 3-fold and more insulin codld improve FPG and IPGTT in db/db mice （P〈0.05）. Conclusion The rAd-p53 can improve FPG,IPGTT and C- peptide in HFD/STZ mice which may be associated with ameliorative β-cell function.

关 键 词：HFD/STZ鼠 db/db鼠 重组人P53腺病毒 糖尿病 2型 

分 类 号：R587.1[医药卫生—内分泌]