冰砂糖寿组培快繁研究  

Tissue Culture and Rapid Propagation of Haworthia kegazato

作　　者：牟豪杰[1] 王燕[1] 吕永平[1] 汪一婷[1] 李海营[1] 

机构地区：[1]浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021

出　　处：《安徽农业科学》2016年第32期140-141,157,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探讨冰砂糖寿组培快繁的最佳条件。[方法]以冰砂糖寿幼嫩花序为外植体,研究冰砂糖寿组培快繁技术。[结果]于MS+5 mg/L 6-BA+0.5 mg/L IBA+30 g/L蔗糖+7g/L琼脂的诱导培养基上成功诱导出愈伤组织,在分化培养基MS+0.3 mg/L6-BA+30 g/L蔗糖+7 g/L琼脂上继代培养可分化成不定芽,将不定芽转接到增殖培养基MS+0.3 mg/L 6-BA+0.1 mg/LNAA+30 g/L蔗糖+7 g/L琼脂上增殖速度较快,增殖系数达3.79。将其移栽至营养基质(混合泥炭∶粗砂=1∶1)中,移栽成活率可达95%。[结论]建立了冰砂糖寿花序再生及快繁体系,为其规模化生产提供理论依据。[Objective]The aim was to study tissue culture and rapid propagation of Haworthia kegazato. [Method]With young inflorescence of Haworthia kegazato as explant,tissue culture and rapid propagation of Haworthia kegazato was studied. [Result]Embryogenic callus was initiated from Haworthia comptoniana × H. correcta cv. ＇Aboukyuu inflorescence cultured on MS medium supplemented with 5 mg / L 6-BA,0. 5 mg / L IBA,30 g / L sucrose,and 7g / L agar. Subculture of these embryogenic calli on the MS ＋ 0. 3 mg / L 6-BA ＋ 30 g / L sucrose ＋ 7 g / L agar medium resulted in shoots and micropropagation of shoots on the MS ＋ 0. 3 mg / L 6-BA ＋ 0. 1 mg / L NAA ＋ 30 g / L sucrose7 g / L agar medium with muitiplication coefficient of 3. 79. The transplanting survival rate was 95% in nutritional substrates.[Conclusion]A subsequent plant regeneration from inflorescence callus of Haworthia kegazato were established,to provide theoretical basis for large-scale production.

关 键 词：冰砂糖寿 愈伤组织 不定芽 

分 类 号：S682.33[农业科学—观赏园艺]