犬急性心房颤动时心房内皮超微结构的变化（英文）  

Changes of atrial endothelial ultrastructure during acute atrial fibrillation in canines

作　　者：潘为涛[1] 陈国桢[2] 蔡诺蔚 吴立春[3] 郑伯仁[1] 

机构地区：[1]福州市第二医院心内科,福建福州350007 [2]福建医科大学附属第二医院心内科 [3]岳阳市第二人民医院ICU

出　　处：《心血管康复医学杂志》2016年第6期561-564,共4页Chinese Journal of Cardiovascular Rehabilitation Medicine

摘　　要：目的:研究犬急性心房颤动时心房内皮细胞超微结构的变化,探讨房颤左房血栓形成的可能机制。方法:取16只健康成年杂种犬,随机均分为空白对照组(仅接受开胸手术而不起博)和快速心房起搏(RAP)组(建立急性房颤模型)。分别取两组犬的左心耳、右心耳部位心肌组织,进行苏木素伊红(HE)染色,在普通光镜下观察心肌细胞形态学改变;在电镜下观察心耳内皮细胞形态学改变。结果:(1)RAP组所有犬均成功诱发出阵发性房颤;(2)普通光镜下两组的心耳、心内膜组织在形态学上无显著差异;(3)透射电镜下,RAP组心耳组织内皮细胞呈现不同程度的不完整,部分脱落,而空白对照组内皮细胞层完整,未见坏死、脱落,与空白对照组比较,RAP组内皮细胞不完整性(12.5%比75.0%)显著增加(P=0.041)。结论:急性房颤时内皮细胞的超微结构已经发生改变,这可能与房颤时附壁血栓形成有关。Objective： To study changes of ultrastructure of atrial endothelial ceils during acute atrial fibrillation （AF） in canines, and explore the possible mechanism of AF left atrial thrombosis. Methods： A total of 16 healthy adult mongrel canines were randomly and equally divided into blank control group （only received thoracotomy without pa- cing） and rapid atrial pacing （RAP） group （established acute AF model）. Myocardial tissue of left and right append- age were taken from two groups and received hematoxylin eosin （HE） staining, then myocardial cell morphological changes was observed under ordinary light microscope; morphological changes of appendage endothelial cells was ob- served under electron microscope. Results ： （1） Paroxysmal AF was successfully induced in all canines of RAP group; （2） There were no significant difference in morphology of appendage and endocardial tissue under ordinary light mi- croscope between two groups; （3） Under transmission electron microscope, endothelium cells of appendage tissue presented defect of different extent, and some shedding in RAP group; while endothelial cell layer was complete with absence of necrosis and shedding in blank control group. Compared with blank control group, there was signifi- cant rise in endothelial cell incompleteness （12.5% vs. 75.0%） in RAP group, P=0. 041. Conclusion： When acute atrial fibrillation occurs, endothelial cell ultrastructure has already changed, which may be related to thrombosis ad- hered to wall during atrial fibrillation.

关 键 词：心房颤动 内皮细胞 肌细胞 心脏 

分 类 号：R541.75[医药卫生—心血管疾病]