分子印迹固相萃取技术测定血清和尿液中16种β-受体激动剂药物  

Molecularly Imprinted Solid Phase Extraction for Multiresidue analysis of 16 β-agonists in Sheep Serum and Urine

作　　者：李婷婷[1] 李溱[2] 李藏兰[1] 贺平丽[1] 

机构地区：[1]中国农业大学动物科学技术学院,动物营养学国家重点实验室,北京100193 [2]中国农业大学生物学院,植物生理学与生物化学国家重点实验室,北京100193

出　　处：《中国畜牧杂志》2016年第23期73-77,共5页Chinese Journal of Animal Science

基　　金：公益性行业(农业)科研专项项目(201203088)

摘　　要：本研究建立了利用分子印迹固相萃取前处理技术结合液相色谱-串联高分辨质谱技术检测绵羊血清和尿液中的16种β-受体激动剂类药物的方法。尿液和血清样品经乙酸铵缓冲液提取,分子印迹固相萃取小柱富集和净化,Atlantis T_3C_（18）色谱柱分离,最后采用质谱一级全扫和数据依赖型二级扫描模式进行定性和定量分析。结果表明：16种药物的线性范围在0.01～50 ng/m L,检出限为0.005～0.153 ng/m L。不同添加浓度下,16种β-受体激动剂在血清和尿样中的添加回收率为62%～115%,相对偏差小于15%。A highly sensitive method for the determination of 16 β-agonists in sheep serum and urine samples was achieved based on molecularly imprinted polymer （MIP） solid phase extraction methodology combined with ultra high performance liquid chromatography-high resolution mass spectrometry （UHPLC-HRMS）. In this study, ammonium acetate buffer was used for sample extraction, and MIP solid phase extraction technology was employed for further sample purification. Then, samples were analyzed using UHPLC-HRMS. Chromatographic separation was achieved with a Waters Atlantis T3 C18 column. Full-scan and data-dependent MS/MS scan mode was applied for qualitative and quantitative analysis. It showed that the linear range of 16 β-agonists was ranged from 0.01-50 ng/mL The limit of detection of 16 β-agonists ranged from 0.005-0.153 ng/mL. Under different spiked levels, the recoveries of 16 β- agonists of were between 62%-115% with the relative deviations less than 15%.

关 键 词：β-受体激动剂 分子印迹固相萃取 液相色谱-串联高分辨质谱 血清 尿液 

分 类 号：S816.7[农业科学—饲料科学]