康复新液对TNBS诱导大鼠溃疡性结肠炎治疗作用的研究  

Experiment Study of Treatment Effects of Kangfuxin Solution on TNBS-Induced Ulcerative Colitis

作　　者：胡婷婷[1] 饶朝龙[1] 耿福能 邓青秀[1] 谢晓芳[1] 

机构地区：[1]成都中医药大学,四川成都611137 [2]四川好医生攀西药业有限责任公司,四川西昌615000

出　　处：《中国民族民间医药》2016年第22期10-14,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：国家自然科学基金项目(J1310034);成都中医药大学中药基础基地科研能力提高项目;中药药理四川省科技创新研究团队(2014TD009);中药资源四川省科技创新研究团队(2016TD0028)

摘　　要：目的:观察康复新液对2,4,6-三硝基苯磺酸(TNBS)诱导的溃疡性结肠炎模型大鼠的治疗作用。方法:将60只SD大鼠随机分为空白对照组(等体积蒸馏水)、模型对照组(等体积蒸馏水)、柳氮磺吡啶组(SASP,0.6g/kg)、康复新液高、中、低剂量组(20、10、5m L/kg),每组10只,雌雄各半。采用TNBS灌肠造模,造模后第3天开始灌胃给药,连续12d,给药结束后各组取材进行HE染色,并用ELISA法测定结肠组织中髓过氧化物酶(MPO)、肿瘤坏死因子α(TNF-α)、白介素-1β(IL-1β)和白介素-10(IL-10)的水平。结果:造模后大鼠结肠呈明显溃疡样损伤;与空白对照组相比较,模型对照组大鼠结肠组织中促炎相关因子MPO、TNF-α和IL-1β的含量升高(P<0.05),抗炎因子IL-10含量降低(P<0.05);与模型对照组比较,康复新液高剂量组大鼠结肠组织损伤减轻,高、中、低剂量组均能降低大鼠结肠组织中MPO、TNF-α含量(P<0.05),也有降低IL-1β含量和升高IL-10含量的作用趋势。结论:康复新液对TNBS诱导的大鼠结肠炎具有良好的治疗效果,机制可能与调节抗炎因子和促炎因子之间的平衡作用有关。Objective To observe the therapeutic effects of Kangfuxin solution on rat model of ulcerative colitis induced by TNBS. Method sixty SD rats were randomly divided into six groups：nomal control groups,model groups,SASP groups,high-dose Kangfux-in groups,medium-dose Kangfuxin groups and low-dose Kangfuxin groups.Each groups have 10 rats including 5 male rats and 5 female rats.TNBS was instilled into the colon of rats to induce ulcerative colitis.3 days after modeling,every groups were given rele-vant drugs once a day for 12d.On the day 12,after administration,the rats were sacrificed to collect colon specimens for HE staining. Elisa was performed to detect the levels of MPO,TNF-α,IL-1βandIL-10 in colon specimens of rat.Result Compared with nomal control groups,the contents of anti-inflammatory factors MPO,TNF-α,IL-1βin colon specimens of rats were significantly higher （P〈0.05）,pro-inflammatory factor IL-10 was significantly reduced （P〈0.05）.Kangfuxin solution can reduce the contents of MPO and TNF-α in colon specimens of rats （P〈0.05）and has a certain influence on the IL-1βand IL-10.Conclusion Kang-fuxin solution has a good therapeutic effect on rat model of ulcerative colitis induced by TNBS and it’s mechanism may be related to adjust the balance between anti-inflammatory factors and pro-inflammatory factors.

关 键 词：康复新液 2  4  6-三硝基苯磺酸 溃疡性结肠炎 抗炎作用 

分 类 号：R285.5[医药卫生—中药学]