车前草醇提物治疗肾草酸钙结石的作用及其机制  

Antiurolithiasic effects of ethanol extract from Plantaginis Herba on calcium oxalate nephrolithiasis in rats

作　　者：吕昂[1] 范新[1] 苏倩 余陈欢[3] 钟宇森[3] 张欢欢[3] 

机构地区：[1]杭州市第一人民医院药学部 [2]杭州市妇产科医院药学部 [3]浙江省医学科学院浙江省实验动物与安全性研究重点实验室

出　　处：《中国临床药理学与治疗学》2016年第11期1239-1245,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省中医药科技计划项目(2013ZA103)

摘　　要：目的:观察车前草醇提物对大鼠肾草酸钙结石形成的影响,并探讨其初步作用机制。方法:采用1%乙二醇联合2%氯化铵诱导大鼠肾草酸钙结石模型。收集各组大鼠尿量;采用化学比色法,检测各组大鼠尿中尿草酸(Ox)、镁离子(Mg^(2+))、肌酐(Cr)含量,血清及肾组织中钙离子(Ca^(2+))、BUN、Mg^(2+)、Cr含量;采用荧光实时定量PCR和蛋白印迹法,分别检测各组大鼠肾组织NOX4、bikunin、Nrf2、HO-1、NQO1和γ-GCS mRNA和蛋白表达水平。结果:与模型组相比,车前草醇提物能显著提高大鼠尿量,提高尿液Ox、Mg^(2+)、Ca^(2+)排泄量,降低血清和肾组织中Ca^(2+)、BUN、Mg^(2+)和Cr含量;此外还能降低肾组织NOX4蛋白表达,提高肾组织bikunin、Nrf2、HO-1、NQO1及γ-GCS mRNA和蛋白表达水平。结论:车前草醇提物可通过促进尿液排泄和上调bikunin、Nrf2/ARE通路相关因子表达,抑制大鼠肾草酸钙结石的形成。AIM： To investigate the effect and its possible mechanism of ethanol extract from Plantaginis Herba （PHEE） on the formation of calcium oxalate stone in rats. METHODS： Oral administration with 1% ethylene glycol and 2% ammonium chloride were performed to induce rat urolithiasis. After 4 weeks treatment, the urine volume, urine 0x, Mg2＋, serum and renal tissue Ca2＋, BUN, Mg2＋, Cr content while the expression of NOX4, bikunin, Nrf2, HO-1, NQO1 and γ-GCS mRNA were detected by qRT-PCR. RESULTS： Compared with the model group, PHEE could significantly increase urine volume and increase the total levels ofurine Ox Mg2. Ca2＋ lower the levels of Ca2＋ BUN, Mg2＋ , Cr in serum and renal tissue ; increase the mRNA and protein expression of bikunin, Nrf2, HOd ,NQ01 and γ-GCS but reduce NOX in renal tissue. CONCLUSION： PHEE can effectively in- hibit the formation of calcium oxalate stone in rats via increasing the volume of urine as well as up-reg- ulation of bikunin and Nrf2/ARE pathways.

关 键 词：车前草 大车前苷 肾结石 BIKUNIN 氧化应激 

分 类 号：R965.2[医药卫生—药理学]