乳源β-Casein-125肽的基本特征及促B淋巴细胞增殖作用  

Basic characteristics of a human milk-derived peptide β-Casein-125 and its effect on B lymphocyte proliferation

作　　者：刘贵友[1] 万俊[2] 肖文[1] 

机构地区：[1]江苏第二师范学院生命科学与化学化工学院,江苏南京210013 [2]南京市妇幼保健院,江苏南京210004

出　　处：《南京工业大学学报（自然科学版）》2016年第6期130-134,共5页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：江苏省自然科学基金(BK20151466)

摘　　要：利用Uniprot、SABLE、ProtParam tool等在线数据库分析β-Casein-125肽生物学特征;利用质谱定量技术检测β-Casein-125肽在初乳、过渡乳和成熟乳中的含量变化;利用细胞增殖实验方法揭示β-Casein-125肽在促进B淋巴细胞增殖中的作用。结果表明:β-Casein-125肽的稳定性系数为111.43,脂肪指数和亲水性分别为111.43和0.471,属于疏水性稳定型多肽。质谱定量分析发现:β-Casein-125肽含量随泌乳时间变化含量显著降低。β-Casein-125肽不仅可以顺利进入小鼠B淋巴细胞,并且可显著促进细胞增殖。β-Casein-125肽具有高稳定性和疏水性的特征,可以促进淋巴细胞增殖,提示该肽可能在新生儿免疫系统建立中发挥重要作用。The biologic characteristics of β-Casein-125 peptide were analyzed by Uniprot, SABLE, ProtParam tool and other online databases. The content ofβ-Casein-125 in colostrum, transition milk and mature milk was mesured by mass spectrometry technology. Furthermore, the effect of β-Casein-125 peptide in B lymphocyte proliferation was evaluated by MTT assay. The online databases showed that stability coefficient, aliphatic index and grand average of hydropathicity of β-Casein-125 were 111.43, 111.43 and 0.471, respectively. It indicated that β-Casein-125 is a hydrophobic stable peptide. The content of β-Casein-125 was significantly decreased with lactation time, detected by mass spectrometry technology. Moreover, β-Casein-125 could across the cell membrane of mouse B lymphocytes, and promote their proliferation. β-Casein-125, a hydrophobic peptide with high stability, could promote＆nbsp;lymphocyte proliferation. It was indicated thatβ-Casein-125 played a critial role in promoting the neonatal immune system maturation.

关 键 词：母乳 β- Casein-125肽 B淋巴细胞 增殖 

分 类 号：Q71[生物学—分子生物学]