水环境游离DNA浓度的初步探讨  

A Preliminary Study on the Concentration of Free DNA in Water Environment

作　　者：李蕊[1] 陈珍珠[1] 田飞[1] 沈彦婷[1] 葛芹玉[2] 

机构地区：[1]东南大学学习科学研究中心 [2]东南大学生物科学与医学工程学院

出　　处：《南京晓庄学院学报》2016年第6期86-88,109,共4页Journal of Nanjing Xiaozhuang University

基　　金：国家自然科学基金面上项目(61271055)

摘　　要：水环境DNA的研究及应用近年来逐渐被大家所认识.为了探讨水环境DNA浓度是否受季节以及地理位置的影响,该文利用实时荧光定量PCR技术对同一位置不同季节以及同一季节不同位置的南京市玄武湖水中游离DNA的浓度进行了比较与分析.结果发现,不同季节的水样中DNA会存在一定的差异,但是当温度上升到一定水平之后,水环境DNA的浓度便不再受温度的影响而变化;此外,湖水中不同位置的DNA浓度也存在一些差异.可见,水环境DNA的浓度能够较好反映特定水体的季节变化和位置信息,为相关生态环境保护和物种资源调查提供了新的方法和思路.The detection and application of environment DNA （ eDNA ） in freshwater has been gradually recog-nized. In order to investigate the relationship between the eDNA concentration and seasons as well as other environ-ment condition, we detected and analyzed the eDNA concentration at a fixed position in different seasons and at dif-ferent positions in the same season in Xuanwu Lake in Nanjing. Results showed that the eDNA concentration in-creased along with the temperature, but there were no significant changes when the temperature reached a certain level. There existed some differences in the eDNA concentration at different positions. It is suggested that the con-centration of DNA in water environment can better reflect the seasonal change, and this provides a novel approach and idea for the related environmental protection and for the investigation of biological species resources.

关 键 词：水环境DNA 温度 季节 地理位置 玄武湖 

分 类 号：X832[环境科学与工程—环境工程]