姜黄素减轻谷氨酸对PC12细胞的氧化应激损伤研究  

Curcumin attenuates glutamate-induced oxidative injury in PC12 cells

作　　者：郭琪[1] 薛小荣[1] 胡斌[1] 李蓓[1] 李艳[1] 王少科[1] 

机构地区：[1]西安市第四医院药剂科,710004

出　　处：《中华神经医学杂志》2016年第12期1212-1216,共5页Chinese Journal of Neuromedicine

摘　　要：目的探讨姜黄素对暴露于谷氨酸中的PC12细胞的保护作用。方法将PC12细胞分为4组，分别为对照组（正常培养）、谷氨酸损伤组（暴露于含15mmol／L谷氨酸的DMEM培养基中以模拟神经元的氧化应激损伤）、姜黄素保护组（暴露于含10μmol／L姜黄素和15mmol／L谷氨酸的DMEM培养基中）和姜黄素单独处理组（暴露于含10μmol／L姜黄素的DMEM培养基中）。细胞孵育24h后。采用MTT法检测细胞活力，采用相差显微镜观察细胞形态，采用比色法检测乳酸脱氢酶（LDH）释放量和胞内活性氧（ROS）含量，采用Western blotting检测凋亡相关蛋白Bax、Bcl-2表达水平。结果与对照组相比，谷氨酸损伤组细胞活力下降，形态受损，LDH释放量增加，胞内ROS含量增加，凋亡蛋白Bax表达水平增高，抗凋亡蛋白Bcl-2表达水平降低，差异均有统计学意义（P＜0．05）；与谷氨酸损伤组相比，姜黄素保护组细胞活力上升，形态较完整，LDH释放量减少，胞内ROS含量减少，Bax表达水平降低，Bcl-2表达水平增高，差异均有统计学差异（P＜0．05）；与对照组相比，姜黄素单独处理组的上述指标差异均无统计学意义（P＞0．05）。结论姜黄素可显著减轻谷氨酸对PC12细胞的氧化应激损伤。Objective To investigate the curcumin （Cur）-induced protective effect on PC12 cells exposed to glutamate （Glu）. Methods PC12 cells were exposed to the medium containing high-level glutamate to mimic oxidative injury of neuronal cells. The PC12 cells were divided into control group （cells cultured in normal medium）, Glu treatment group （cells cultured in the medium containing 15 mmol/L glutamate）, Cur＋Glu treatment group （cells cultured in the medium containing 10 μmol/L curcumin plus 15 mol/L glutamate） and Cur treatment group （cells cultured in the medium containing 10 μmol/L curcumin alone）. After 24 h incubation, MTT method was used to assess the cell viability; specific reagent kit was taken to evaluate the lactic dehydrogenase （LDH） release and intracellular reactive oxygen species （ROS） level; phase contrast microscope was taken to record the cell morphology; and Western blotting was used to determine the expressions of apoptosis-associated proteins （Bax and Bcl-2）. Results As compared with the control group, the Glu treatment group had decreased cell viability, increased LDH release, injured cell morphology, enhanced intracellular ROS level, up-regulated Bax expression and down-regulated Bcl-2 expression, with significant differences （P〈0.05）. As compared with those in the Glu treatment group, significantly meliorated cell viability, decreased LDH release, protected cell morphology, reduced ROS level, down-regulated Bax expression and up-regulated Bcl-2 expression in the Cur＋Glu treatment group were noted （P〈0.05）. The above indexes between the control group and Cur treatment group showed no significant differences （P〉0.05）. Conclusion Cur attenuates the glutamate-induced oxidative injury in PC 12 cells.

关 键 词：姜黄素 PC12细胞 谷氨酸 氧化应激损伤 神经保护 

分 类 号：R285[医药卫生—中药学]