人参二醇的微生物转化及抗HSC-T6细胞活性研究  

Microbial Transformation of 20(R)-Panaxadiol and Their Activity Against HSC-T6 Cells

作　　者：朱梦月 雷静[1] 周垚[1] 戴秋阳 朱腊梅[1] 陈广通[1] 

机构地区：[1]南通大学药学院,江苏南通226001

出　　处：《常州大学学报（自然科学版）》2016年第6期23-27,共5页Journal of Changzhou University：Natural Science Edition

基　　金：江苏省高等学校大学生创新创业训练计划项目(201610304075Y);南通市科技项目(MS12015044)

摘　　要：研究了20（R）-人参二醇衍生物转化及其抗HSC-T6细胞活性。利用微生物刺囊毛霉Mucorspinosus AS 3.3450对20（R）-人参二醇进行转化,采用硅胶柱色谱、制备型高效液相色谱技术分离、纯化产物,并通过波谱数据分析确认了产物结构。采用噻唑蓝（MTT）法测试产物抗HSC-T6肝星状细胞活性。共分离鉴定出了5个化合物：3-羰基-20（R）-人参二醇（1）,3-羰基-7β,24α-二羟基-20（R）-人参二醇（2）,3-羰基-7β,24β-二羟基-20（R）-人参二醇（3）,3-羰基-15α,24α-二羟基-20（R）-人参二醇（4）,3-羰基-15α,24β-二羟基-20（R）-人参二醇（5）。MTT结果显示,化合物1~3抑制HSC-T6细胞增值的作用强于20（R）-人参二醇。To investigate the derivatives of 20（R）-panaxadiol and their activities against HSC-T6 cells,20（R）-panaxadiol was treated by Mucorspinosus AS 3.3450.All products were isolated by silica gel chromatography and purified by preparative HPLC.On the basis of spectral data,structures were elucidated.The activity against HSC-T6 cells was measured by MTT method.Five biotransformation products were obtained and identified as 3-oxo-20（R）-panaxadiol（1）,3-oxo-7β,24α-dihydroyl-20（R）-panaxadiol（2）,3-oxo-7β,24β-dihydroyl-20（R）-panaxadiol（3）,3-oxo-15α,24α-dihydroyl-20（R）-panaxadiol（4）,3-oxo-15α,24β-dihydroyl-20（R）-panaxadiol（5）.MTT assay revealed that compounds 1-3 showed higher inhibitory activity against HSC-T6 cells than 20（R）-panaxadiol.

关 键 词：人参二醇 微生物转化 HSC-T6细胞 抗肝纤维化 

分 类 号：R284.1[医药卫生—中药学]