新型吖啶衍生物的合成及其与DNA相互作用研究  

Synthesis of an Acridine Derivative and Its DNA Interaction Studies

作　　者：邵莺[1] 童晶晶[1] 吴祝宏 黄磊[1] 孙小强[1] 

机构地区：[1]常州大学石油化工学院,江苏常州213164

出　　处：《常州大学学报（自然科学版）》2016年第6期38-43,共6页Journal of Changzhou University：Natural Science Edition

基　　金：国家自然科学基金青年基金项目资助(21302015)

摘　　要：用两种方法从便宜的原料N-苯基邻氨基苯甲酸出发,合成了新型的,具有荧光活性的吖啶衍生物9-(1-氮杂环丁基)吖啶Ⅲ。用核磁1 H NMR、13 C NMR、高分辨质谱HR MS以及熔点等手段,对每种化合物或者中间体,进行了必要的结构表征。并通过紫外滴定法、荧光滴定法等手段,结合溴乙锭荧光探针等实验,得出了化合物Ⅲ与DNA以嵌插模式结合,并计算得到了相应的结合常数,结合位点。The novel fluorescent acridine derivative,9-（azetidin-1-yl））acridine Ⅲ was synthesized using two methods from cheap 2-（phenylamino）benzoic acid as raw material.All the synthetic intermediates or target compounds were successfully prepared and confirmed with melting points,1 H NMR,13 C NMR and HRMS spectra.In addition,UV-vis titration,fluorescence titration,and EB fluorescent probe assays were used to study the interaction between the compound Ⅲ and DNA.The results give the binding constant and number of binding sites,and indicate that the compound Ⅲis binding to DNA with the intercalation mode.

关 键 词：吖啶衍生物 DNA相互作用 紫外滴定 荧光滴定 嵌插模式 

分 类 号：O621.3[理学—有机化学]