软枣猕猴桃离体快速繁殖技术  

Micropropagation in vitro of Actinidia arguta

作　　者：赵芮[1] 赵春莉[1] 安佰义[1] 董然[1] 王克凤 顾德峰[1] 

机构地区：[1]吉林农业大学园艺学院,长春130118 [2]长春科技学院,长春130600

出　　处：《湖北农业科学》2016年第19期5007-5010,共4页Hubei Agricultural Sciences

基　　金：农业部"948"项目2013(z32);吉林省科技支撑计划项目(20100259)

摘　　要：以软枣猕猴桃（Actinidia arguta）枝条萌发的幼芽为外植体,以MS为基础培养基,添加不同质量浓度的细胞分裂素6-BA、生长素NAA和IBA,探索了软枣猕猴桃的离体快速繁殖技术。结果表明,软枣猕猴桃丛生芽增殖的最佳培养基为MS＋2.0 mg/L 6-BA＋0.2 mg/L IBA＋30 g/L蔗糖,p H为5.8~6.0,在此条件下,其平均增殖系数为4.69,且诱导的无菌苗健壮;最适生根培养基为1/2MS＋0.4 mg/L IBA＋30 g/L蔗糖＋2.0 g/L活性炭,生根率达100%,且根系健壮、数量繁多。Using bud of Actinidia arguta branches germination as explants,with MS as basic culture medium by adding different mass concentration cytokinin 6-BA, auxin NAA and IBA,the micropropagation in vitro technology of A. arguta was explored. The results showed that the optimum proliferation medium of shoot cluster was MS＋2.0 mg / L 6-BA＋0.2 mg / L IBA＋30g / L sucrose,and p H was 5.8 to 6.0. Under this condition,the average multiplication coefficient was 4.96 with vigorous aseptic seedling. The best rooting medium was 1 / 2MS＋0.4 mg / L IBA＋30 g / L sucrose＋2.0 g / L activated carbon,at these condition,the rooting rate was 100%,and the roots were well developed and had great quantity.

关 键 词：软枣猕猴桃(Actinidia arguta) 离体快速繁殖 丛生芽增殖 

分 类 号：S663.4[农业科学—果树学]