SSR分子标记技术在汉青一号萝卜种子纯度鉴定中的应用  

Application of SSR Molecular Marker Technique in Seed Purity Identification of Radish Hybrid HanQing NO.1

作　　者：张小康[1] 朱运峰[1] 陈玉霞[2] 徐长城[1] 张雪丽[1] 熊秋芳[1] 

机构地区：[1]武汉市蔬菜科学研究所,武汉430345 [2]湖北省农业科学院农产品加工与核农技术研究所,武汉430064

出　　处：《湖北农业科学》2016年第19期5069-5071,共3页Hubei Agricultural Sciences

基　　金：武汉市农业科学研究院2015集成创新项目

摘　　要：选用120对引物在汉青一号萝卜(Raphanus sativus L.cv.Han Qing NO.1)亲本间进行PCR扩增,从中筛选出3对引物10、30、63,在双亲本间能够扩增出差异互补的条带,利用3对引物对汉青一号萝卜杂种1代群体进行纯度鉴定。鉴定结果纯度为95.1%,与同批次种子的田间鉴定结果 96.0%相近,说明应用SSR分子标记技术实施萝卜杂种1代种子的纯度鉴定是可行并可靠的。120 pairs of SSR primers were selected for PCR amplification between the parental lines of radish hybrid（Raphanus sativus L.） Han Qing-No.1. In which 3 pairs of primers,10,30 and 63 could amplify difference-complementary bands from either of the parents. Using the 3 pairs of primers to identify the purity of Han Qing-No.1, the results showed that the seed purity was 95.1%,which was approximate to the field purity（96% in the same batch of seeds identification）.The conclusion suggested that the application of SSR molecular markers technique could be feasible and reliable for the purity identification of radish hybrid seed.

关 键 词：汉青一号萝卜（Raphanus SATIVUS L.cv.Han QING NO.1） SSR分子标记技术 种子纯度 鉴定 

分 类 号：S338[农业科学—作物遗传育种] S631.1[农业科学—农艺学]