枯草芽孢杆菌BS-8D防治玉米纹枯病的田间试验效果及作用机理  

The Biocontrol of Maize Sheath Blight and Mechanism with Bacillus subtilis BS-8D Strain in the Field

作　　者：毛腾霄 叶华智[2] 秦玉花[3] 

机构地区：[1]成都市食品药品检验研究院,成都610041 [2]四川农业大学农学院,成都611130 [3]成都中医药大学基础医学院,成都610072

出　　处：《湖北农业科学》2016年第20期5252-5255,共4页Hubei Agricultural Sciences

摘　　要：从玉米植株的茎基部叶鞘分离得到枯草芽孢杆菌菌株(Bacillus subtilis BS-8D)。田间小区防病试验结果表明,该菌株对玉米纹枯病有较好的防效。采用抗利福平标记突变菌株BS-8Drif测定其定殖能力,结果显示该菌株在玉米叶鞘上有较好的定殖能力,施用10 d后仍能保持较高密度。对BS-8D菌株拮抗机理研究发现,菌株无菌培养液能抑制玉米纹枯病菌(Rhizoctoia solani)核萌发和菌丝生长。Antagonistic agents of Bacillus subtilis BS-8D was isolated from the sheaths and rhizosphere of corn. The results showed that the strain BS-8D could control effectively corn sheath blight caused by the Rhizoctonia solani in field. BS-8D could colonize, propagate and remain high population at sheaths for more than ten days. The highest colonizing level was reached on 10 th day after inoculation. The mechanism study of BS-8 d antagonism showed that the culture filtrate from BS-8D had a strong inhibiting activity against Rhizoctoia solani sclerotium germination and the growth of mycelia.

关 键 词：枯草芽孢杆菌(Bacillus subtilis) 玉米纹枯病 生物防治 抗菌物质 田间试验 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]