缬草黄酮纯化工艺研究  

Purification Technology of Flavonoids from Valeriana officinalis L.

作　　者：谷政伟 胡铁[2] 黎继烈[1] 李振海[1] 

机构地区：[1]中南林业科技大学经济林培育与利用湖南省2011协同创新中心/经济林培育与保护省部共建教育部重点实验室,长沙410004 [2]广州航海学院国际交流与合作办公室,广州510725

出　　处：《湖北农业科学》2016年第20期5336-5339,5344,共5页Hubei Agricultural Sciences

基　　金：林业公益性行业科研专项(201404608);生物学湖南省"十二五"重点建设学科项目

摘　　要：为探讨缬草(Valeriana officinalis L.)黄酮纯化工艺,以树脂对黄酮的吸附率和解吸率为评价指标,通过静态吸附-解吸试验对6种树脂进行优选,在此基础上开展动态吸附-解吸试验,对上样与洗脱条件进行优化。结果表明,采用聚酰胺柱层析纯化缬草黄酮,在上样液浓度2.0 mg/m L、上样流速1.5 m L/min、上样量20.0 m L、上样液p H 5.5的条件下,用70.0%乙醇洗脱,洗脱流速2.0 m L/min、洗脱剂70.0 m L纯化3次,洗脱液经干燥、检测,发现黄酮纯度由粗品17.58%提高到79.17%。该工艺简单、安全、环保,可得到较高纯度的黄酮,适合纯化缬草黄酮。In order to investigate the purification process of flavonoids from Valeriana officinalis L.,the optimum resin was chosen from 6 kinds of resins by the static absorption-desorption tests and the adsorption and desorption rate of resins to flavonoids were used as index. Based on the above results,the sample and elution conditions were optimized by the dynamic absorption-desorption experiments. Flavonoids from Valeriana officinalis L. were purified by polyamide column chromatography and the optimum sample conditions were concentration 2.0 mg/mL,velocity 1.5 mL/min,amount 20.0 mL and pH value 5.5,the optimal elution conditions were ethanol concentration 70.0%,velocity 2.0 mL/min and amount 70.0 mL. Under the above conditions,the purity of flavonoids was from 17.58% up to 79.17% by three times purification. The above approach is appropriate to purify flavonoids from Valeriana officinalis L. for it is safe,friendly to environment,easy to operate and can gain flavonoids safely with high purity.

关 键 词：缬草(Valeriana OFFICINALIS L.) 黄酮 纯化 

分 类 号：S567.239[农业科学—中草药栽培] R284.2[农业科学—作物学]