动态固相微萃取-气相色谱法测定环境空气中挥发性脂肪酸的研究  

Analysis of Six Volatile Fatty Acids in Air Using Dynamic Solid-phase Microextration-Gas Chromatography

作　　者：孟洁[1,2] 王亘[1] 韩萌[1] 翟增秀[1,2] 耿静[1] 鲁富蕾 

机构地区：[1]天津市环境保护科学研究院国家环境保护恶臭污染控制重点实验室,天津300191 [2]天津迪兰奥特环保科技开发有限公司,天津300191

出　　处：《分析测试学报》2016年第12期1611-1615,共5页Journal of Instrumental Analysis

基　　金：国家重大科学仪器设备开发专项(2012YQ060165)

摘　　要：建立了动态固相微萃取-气相色谱（FID）测定环境空气中挥发性脂肪酸（VFAs）的分析方法,选择乙酸（AA）、丙酸（PA）、丁酸（BA）、异丁酸（iso-BA）、戊酸（VA）和异戊酸（iso-VA）6种代表性VFAs为研究对象,研制了动态萃取装置并建立了动态萃取机制。研究了固相微萃取材料、萃取时间、萃取温度、萃取流速、解析温度及时间对VFAs萃取效率的影响,在此基础上优化了气相色谱法检测条件。在优化条件下,6种脂肪酸的线性范围均达到4个数量级,相关系数均大于0.990。方法的检出限（S/N=3）为1.0-4.7μg/L,相对标准偏差（RSD,n=3）为1.1%-6.8%。与常规方法相比,该方法大大提高了检测范围和检测灵敏度,并成功用于环境空气中挥发性脂肪酸的分析检测。An analytical method for the detection of volatile fatty acids （VFAs） was established. Acetic acid（AA）, propionic acid（PA）, butyric acid（BA）, iso-butyric acid（iso-BA）, valerie acid （VA） and iso-valeric acid（iso-VA） were chosen as the target, and a dynamic solid-phase mieroex- tration（SPME） device was developed. The effects of extraction materials, extraction time, extrac- tion temperature, extraction flow rate, extraction temperature and time of desorption were investiga- ted on the efficiency of SPME extraction, and by which the optimal extraction conditions were deter- mined. Furthermore, test conditions for gas chromatography were optimized, and a new dynamic SPME method was developed for gas chromatography ionization detection （GC - FID ） to determine VFAs with the detection limits of 1.0 -4.7 μg/L. The RSDs for three replicate extractions ranged from 1.1% to 6. 8% , and the calibration curves were linear in the four orders of magnitude, with correlation coefficients （ r2 ） more than 0. 990. The developed method was successfully applied in the determination of trace VFAs in the environment.

关 键 词：挥发性脂肪酸 动态固相微萃取 气相色谱(GC) 空气 

分 类 号：O625.32[理学—有机化学]